Evolution of odorant receptor genes in Ostrinia moths and its involvement in male response to sex pheromone.

性信息素在物种特定的交配行为中起着至关重要的作用，然而，对于负责这些行为的基因发生了什么变化，人们知之甚少。因此，我们对亚洲玉米螟和亚洲玉米螟性信息素反应和合成相关基因进行了比较分析。我们构建了该物种的基因组化石文库，并建立了基于pcr的筛选系统。然后，我们分离了含有编码受体、结合蛋白和性信息素去饱和酶基因的克隆，并确定了基因组序列（O. furnaclis为330kb， O. latipennis为265kb）。

Sex pheromone plays a critical role in species-specific mating behaviors, however, little is known about what changes occur in genes responsible for the behaviors. Thus, we performed a comparative analysis of genes associated with sex pheromone response and synthesis between Ostrinia furnacalis and Ostrinia latipennis. We constructed genomic fosmid libraries of the species, and generated PCR-based screening system for them. Then, we isolated clones that contain genes encoding receptors, binding proteins and desaturase of sex pheromone, and determined genomic sequences (330kb for O. furnaclis and 265kb for O. latipennis)