Isolation of BAC clones containing conserved genes from libraries of three distantly related moths: a useful resource for comparative genomics of Lepidoptera.
Isolation of BAC clones containing conserved genes from libraries of three distantly related moths: a useful resource for comparative genomics of Lepidoptera.
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DOI：
10.1155/2011/165894
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发表时间：
2011
影响因子：
--
通讯作者：
Sahara K
中科院分区：
文献类型：
--
作者：
Yasukochi Y;Tanaka-Okuyama M;Kamimura M;Nakano R;Naito Y;Ishikawa Y;Sahara K
鳞翅目，蝴蝶和蛾，是第二大动物目，包括许多农业害虫。为了促进鳞翅目比较基因组学，我们从三种害虫文库中分离出含有保守和推定的单拷贝基因的BAC克隆，这三种害虫是烟夜蛾、欧洲玉米螟和小菜蛾，具有单倍体染色体数目，n = 31，它们彼此或与家蚕（n = 28）（已测序的模式鳞翅目昆虫）不密切相关。每个物种共分离到108-184个克隆，代表101-182个保守基因。对于79个基因，从两个以上的物种中分离克隆，这将是有用的，作为共同的标记，使用荧光原位杂交（FISH）分析，以及比较多个物种之间的基因组序列。本文提出的基于PCR的克隆分离方法适用于缺乏测序基因组但具有大量cDNA或EST序列的物种。
Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, n = 31, which are not closely related with each other or with the silkworm, Bombyx mori, (n = 28), the sequenced model lepidopteran. A total of 108–184 clones representing 101–182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species. The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.
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影响因子：
4.4
作者：
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