Molecular ecological study of dissemination and reproduction of tree pathogenic fungi

为了了解树木病原真菌的传播和繁殖机制，我们开发了Raffaelea quercivora、taaphrina wiesneri和Perenniporia fraxinea的多态性微卫星标记。利用这些标记，我们研究了这些真菌在严重受损部位的遗传结构。此外，我们还利用T-RFLP分析方法调查了被侵染树木树干内甜蜜环菌（Armillaria tabescens）、平顶灵芝（Ganoderma applanatum）和拉克西菌（P. fraxinea）的分布。我们还利用F-WGA菌丝染色法检测了侵染树木木质部中更细尺度的菌丝分布。

To know the dispersal and reproductive mechanisms of tree pathogenic fungi, we developed polymorphic microsatellite markers of Raffaelea quercivora, Taphrina wiesneri, and Perenniporia fraxinea. Using these markers, we investigated the genetic structure of these fungi in seriously damaged sites. In addition, we investigated distribution of Armillaria tabescens, Ganoderma applanatum, and P. fraxinea within the trunk of infested trees using T-RFLP analysis. We also examined finer scale hypha distribution in the xylem of infested trees using hyphal staining with F-WGA.