Establishment of a method for synthesizing artificial Shiro of Tricholoma matsutake using split-root system

分根系统合成人工松茸四罗方法的建立

• 批准号: 24658131
• 负责人: MATSUSHITA Norihisa
• 金额: $ 2.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658131/
• 关键词: マツタケ; シロ; 外生菌根菌; 分根; 共生; アカマツ; 外生菌根; 根分け; クロマツ

To produce fruiting bodies of Tricholoma matsutake under controlled conditions, it is necessary to develop Shiro which is compact mass of mycelia and mycorrhizae of T. matsutake. In this study, we tried to establish a method for synthesizing artificial Shiro using split-root system. We examined the moisture condition of the seedbed and the age of seedlings to be used for cuttings, and determined the optimal condition for obtaining the cuttings of Japanese red pine and Japanese black pine. In addition, we established the inoculation method of ectomycorrhizal fungi for the split-root system. It was possible to investigate the translocation of nitrogen from mycorrhizal root systems to nonmycorrhizal root systems using the split-root system of Japanese red pine. Therefore, we considered that the sprit-root system established in this study to be very effective in studying the ectomycorrhizal symbiosis.

为了在受控条件下生产松茸子实体，必须培养由T.松茸。本研究试图建立一种利用分根系统合成人工四郎的方法。通过对扦插育苗的苗床水分条件和苗木年龄的研究，确定了获得日本赤松和日本黑松扦插苗的最佳条件。此外，还建立了分根系统外生菌根真菌的接种方法。利用红松分根系统研究氮素从菌根根系向非菌根根系的转移是可能的。因此，我们认为本研究建立的芽-根系统是研究外生菌根共生关系的有效方法。

项目成果

アカマツ挿し木分根培養系における窒素転流への施肥および外生菌根菌接種の影響

施肥和外生菌根真菌接种对日本红松插条根系培养系统氮转运的影响

• 发表时间: 2014
• 作者: 棗悠紀・松下範久

マツタケ外生菌根共生系の生態

松茸外生菌根共生系统的生态学

• 发表时间: 2013
• 作者: 棗悠紀・松下範久;棗悠紀・松下範久;松下範久
• 通讯作者: 松下範久

マツタケのシロの拡大に伴う外生菌根菌相および土壌菌類相の変化

松茸扩张导致外生菌根真菌和土壤真菌的变化

• 发表时间: 2013
• 作者: 棗悠紀・松下範久;棗悠紀・松下範久;松下範久;松下範久・小松隆平・竹内嘉江・宝月岱造
• 通讯作者: 松下範久・小松隆平・竹内嘉江・宝月岱造