Establishment of a method for synthesizing artificial Shiro of Tricholoma matsutake using split-root system

为了在受控条件下生产松茸子实体，必须培养由T.松茸。本研究试图建立一种利用分根系统合成人工四郎的方法。通过对扦插育苗的苗床水分条件和苗木年龄的研究，确定了获得日本赤松和日本黑松扦插苗的最佳条件。此外，还建立了分根系统外生菌根真菌的接种方法。利用红松分根系统研究氮素从菌根根系向非菌根根系的转移是可能的。因此，我们认为本研究建立的芽-根系统是研究外生菌根共生关系的有效方法。

To produce fruiting bodies of Tricholoma matsutake under controlled conditions, it is necessary to develop Shiro which is compact mass of mycelia and mycorrhizae of T. matsutake. In this study, we tried to establish a method for synthesizing artificial Shiro using split-root system. We examined the moisture condition of the seedbed and the age of seedlings to be used for cuttings, and determined the optimal condition for obtaining the cuttings of Japanese red pine and Japanese black pine. In addition, we established the inoculation method of ectomycorrhizal fungi for the split-root system. It was possible to investigate the translocation of nitrogen from mycorrhizal root systems to nonmycorrhizal root systems using the split-root system of Japanese red pine. Therefore, we considered that the sprit-root system established in this study to be very effective in studying the ectomycorrhizal symbiosis.