Studies for molecular mechanism of meiotic-competence-acquisition in porcine growing oocytes

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Mammalian growing oocytes cannot resume CDC2-activity-mediated meiotic maturation, even if they are grown up to 90% of their final size. In the present study, the molecular mechanisms of this incompetence were analyzed using porcine oocytes.As the results, it was revealed that porcine growing oocytes was inhibited their meiotic maturation by the two mechanisms, one was the insufficient CDC2 amount and the another one was the suppression of CDC2 activation by the regulation of PKA localization. In addition, the differences of PKA regulatory subunits (PKA-Rs) and A kinase anchoring proteins (AKAPs), which were involved in the control of PKA localization, were suggested between fully-grown and growing oocytes. I provided new insight that the change of PKA regulation mechanism during oocyte growth phase was the principle for the acquisition of meiotic competence in mammalian growing oocytes.