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Studies for molecular mechanism of meiotic-competence-acquisition in porcine growing oocytes

猪生长卵母细胞减数分裂能力获得的分子机制研究

基本信息

批准号：
22380147
负责人：
NAITO Kunihiko
金额：
$ 12.65万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010-04-01 至 2013-03-31
项目状态：
已结题

项目摘要

Mammalian growing oocytes cannot resume CDC2-activity-mediated meiotic maturation, even if they are grown up to 90% of their final size. In the present study, the molecular mechanisms of this incompetence were analyzed using porcine oocytes.As the results, it was revealed that porcine growing oocytes was inhibited their meiotic maturation by the two mechanisms, one was the insufficient CDC2 amount and the another one was the suppression of CDC2 activation by the regulation of PKA localization. In addition, the differences of PKA regulatory subunits (PKA-Rs) and A kinase anchoring proteins (AKAPs), which were involved in the control of PKA localization, were suggested between fully-grown and growing oocytes. I provided new insight that the change of PKA regulation mechanism during oocyte growth phase was the principle for the acquisition of meiotic competence in mammalian growing oocytes.

哺乳动物发育中的卵母细胞不能恢复CDC2活性介导的减数分裂成熟，即使它们发育到最终大小的90%。本研究以猪卵母细胞为研究对象，分析了猪卵母细胞功能不全的分子机制。结果表明，猪成熟卵母细胞的减数分裂成熟受到两种机制的抑制，一种是CDC2含量不足，另一种是通过调节PKA的定位来抑制CDC2的激活。此外，还发现了参与PKA定位调控的PKA调节亚基(PKA-Rs)和A激酶锚定蛋白(AKAPs)在成熟卵母细胞和发育中卵母细胞中的差异。提出了卵母细胞发育过程中PKA调控机制的变化是哺乳动物发育中卵母细胞获得减数分裂能力的基本原理。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The regulation mechanism of phosphorylation of CDC2 threonine 161 during porcine oocyte maturation

猪卵母细胞成熟过程中CDC2苏氨酸161磷酸化的调控机制

DOI：
发表时间：
2012
期刊：
影响因子：
0
作者：
Fujii W;Nishimura T;Kano K;Koji Sugiura;K Naito K
通讯作者：
K Naito K

Cdk7 and Cyclin H, but not Mat1, are involved in meiotic resumption in porcine immature oocyte.

Cdk7 和 Cyclin H（但不包括 Mat1）参与猪未成熟卵母细胞减数分裂的恢复。

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Kozue Uchio-Yamada;Kyoko Sawada;Kotaro Tamura;Sumie Katayama;Youko Monobe;Yoshie Yamamoto;Atsuo Ogura;Noboru Manabe;築山智之・今井裕;Fujii W
通讯作者：
Fujii W

ブタ成長途上卵における減数分裂能欠損の分子機構

猪蛋发育过程中减数分裂缺陷的分子机制