Challenging research for functional analyses of piRNA/PIWI in mammalian female germ cells

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At least two PIWI mRNA, PIWIL1 and PIWIL2, are present in porcine oocytes and the inhibition of these protein expression results in the meiotic resumption of oocytes under a high cAMP concentration, suggesting the physiological involvement of PIWI in the meiotic arrest of porcine ovarian oocytes. This PIWI function is mediated by the inhibition of cyclin B accumulation and might affect on the APC function, which works on the cyclin B degradation, as no differences are detected in the translational regulations, such as cyclin B mRNA level, Aurora A and CPEB status.