Molecular mechanisms linking energy metabolism and thermoregulation

连接能量代谢和体温调节的分子机制

• 批准号: 22390014
• 负责人: UMEDA Masato
• 金额: $ 11.32万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390014/
• 关键词: 体温調節; エネルギー代謝; 脂質代謝; メタボリックシンドローム; ショウジョウバエ

For ectothermic animals, behavior plays a major role in thermoregulation; a wide variety of animals sense the environmental temperature and move towards to thermally comfortable zone. Although most ectothermic animals do not use metabolically produced heat to regulate body temperature, we found that thermoregulatory behavior of Drosophila is highly dependent on the level of energy metabolism; the increase in mitochondrial oxidative phosphorylation induced preference for the low temperature (Science 323:1740-1743, 2009), while the suppression of energy metabolism by inhibiting insulin signaling caused a warm-seeking thermoregulatory behavior. During the course of identifying molecules involved in the control of energy homeostasis, we found that delta-9 fatty acid desaturase (desat1) expressed in the fat body, a tissue equivalent to mammalian liver and adipose tissue, played a pivotal role in the control of energy metabolism as well as thermoregulatory behavior. Defective expression of desat1 suppressed mitochondrial oxidative phosphorylation, causing a significant decrease in body size and warm-seeking phenotype. In contrast, induced expression of delta-12 fatty acid desaturase (Caenorhabditis elegansFat-2) that produces linoleic acid caused the increase in body size and the low-temperature preference. These observations suggest that the changes in lipid desaturation coordinately regulate mitochondrial energy metabolism and thermoregulatory behavior.

对于变温动物来说，行为在体温调节中起着重要的作用，各种各样的动物都能感知环境温度并向热舒适区移动。虽然大多数变温动物不使用代谢产生的热量来调节体温，但我们发现果蝇的体温调节行为高度依赖于能量代谢水平;线粒体氧化磷酸化的增加诱导了对低温的偏好（Science 323：1740-1743，2009），而通过抑制胰岛素信号传导抑制能量代谢引起了寻温的体温调节行为。在鉴定参与能量稳态控制的分子的过程中，我们发现在脂肪体（相当于哺乳动物肝脏和脂肪组织的组织）中表达的δ-9脂肪酸去饱和酶（desat 1）在控制能量代谢以及体温调节行为中起关键作用。desat 1的表达缺陷抑制线粒体氧化磷酸化，导致身体大小和趋温表型显着减少。相比之下，产生亚油酸的δ-12脂肪酸去饱和酶（秀丽隐杆线虫Fat-2）的诱导表达导致体型增加和低温偏好。这些观察结果表明，脂质去饱和的变化协调调节线粒体能量代谢和体温调节行为。

项目成果

Role of fatty acid remodeling in the control of mitochondrial energy metabolism in Drosophila melanogaster

脂肪酸重塑在果蝇线粒体能量代谢控制中的作用

• 发表时间: 2012
• 作者: Taura M;Suico MA;Koyama K;Komatsu K;Miyakita R;Matsumoto C;Kudo E;Kariya R;Goto H;Kitajima S;Takahashi C;Shuto T;Nakao M;Okada s;Kai H;Masato Umeda
• 通讯作者: Masato Umeda

Molecular mechanisms linking energy homeostasis to behavioral thermoregulation in Drosophila

将果蝇能量稳态与行为温度调节联系起来的分子机制

• 发表时间: 2010
• 作者: Sasaki;T.;N. Kuga;S. Namiki;N. Matsuki and Y. Ikegaya;梅田真郷
• 通讯作者: 梅田真郷

FRMD4A regulates epithelial polarity by connecting Arf6 activation with the PAR complex

• DOI: 10.1073/pnas.0908423107
• 发表时间: 2010-01-12
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Ikenouchi, Junichi;Umeda, Masato
• 通讯作者: Umeda, Masato

トランスポートソームの世界-膜輸送研究の源流から未来へ

运输体的世界 - 从膜运输研究的起源到未来

• 发表时间: 2011
• 作者: Nozu T;Tsuchiya Y;Kumei S;Takakusaki K;Okumura T;山田勝也
• 通讯作者: 山田勝也

Calcium-dependent phospholipids scrambling by TMEM16F.

TMEM16F 扰乱钙依赖性磷脂。

• 发表时间: 2010
• 期刊: Nature.
• 作者: Suzuki J;Umeda M;Sims PJ;Nagata S.
• 通讯作者: Nagata S.