Membrane phospholipid dynamics and cytoskeletal organization: Regulation of actin filament assembly by redistribution of membrane surface phospholipid

膜磷脂动力学和细胞骨架组织：通过膜表面磷脂的重新分布调节肌动蛋白丝组装

• 批准号: 09480201
• 负责人: UMEDA Masato
• 金额: $ 6.08万
• 依托单位: Tokyo Metropolitan Organization for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480201/
• 关键词: phospholipid; cytoskeleton; lipid bilayer; membrane; neuron; actin; yeast; phosphatidylethanolamine; ニューロン; マクチン; 変異体; 変異株

Using a novel peptide probe, Ro09-0198, which specifically forms a tight equimolar complex with phosphatidylethanoalmaine (PE) on biological membranes, we have analyzed the cell surface movement of PE in dividing CHO cells. We found that flip-flop of plasma membrane PE was markedly enhanced at the cleavage furrow during the late telophase of cytokinesis and that cell surface trapping of PE induced by adding the peptide-streptavidin complex to the mitotic cells blocked cytokinesis at the late telophase. Further analysis have shown that the redistribution of plasma membrane phospholipids acts as a regulator of actin filament assembly and play a crucial role in mediating a coordinate movement between plasma membrane and cytoskeleton. Molecules involved in the cross-talk between the phospholipid dynamics and cytoskeletal reorganization have been investigated using yeast and CHO mutant cells which were selected by their altered sensitivity to the PE-binding peptide.

利用一种新型多肽探针Ro09-0198与磷脂酰乙醇胺(PE)在生物膜上形成紧密的等摩尔复合体，分析了PE在CHO细胞分裂过程中的细胞表面运动。我们发现，在胞质分裂末期，质膜PE的翻转在分裂沟处明显增强，而在有丝分裂末期，加入多肽-链霉亲和素复合体引起的PE对细胞表面的捕获阻碍了胞质分裂。进一步的分析表明，质膜磷脂的重新分布是肌动蛋白细丝组装的调节因子，在调节质膜和细胞骨架之间的协调运动中起着至关重要的作用。利用酵母和CHO突变细胞研究了参与磷脂动力学和细胞骨架重组之间的串扰的分子，这些突变细胞是通过对PE结合肽的敏感性改变而被选择的。

项目成果

梅田真郷、榎本和生: "膜融合におけるリン脂質ダイナミクスと細胞骨格制御"生物物理. 21. 18-23 (1999)

Shingo Umeda、Kazuo Enomoto：“膜融合中的磷脂动力学和细胞骨架控制”生物物理学。 21. 18-23 (1999)

榎本和生 ら: "Exposure of phosphatidylethanolamine on the surfau of apoptotic cells" Exp.Cell.Res.232. 430-434 (1997)

Kazuo Enomoto 等人：“凋亡细胞表面暴露磷脂酰乙醇胺”Exp.Cell.Res.232 (1997)。

H.Kobayashi, Y.Sekizawa, S. Shinoda, S.Natori, T.Nakajima, M.Umeda: "Lysenin, a novel bioactive protein isolated from coelomic fluid of the earthworm Eisenia foetida: Structure, secretion and biological activity. Neurobiology: Prospect and Perspectives"Sp

H.Kobayashi、Y.Sekizawa、S. Shinoda、S.Natori、T.Nakajima、M.Umeda：“Lysenin，一种从蚯蚓体腔液中分离出来的新型生物活性蛋白：结构、分泌和生物活性。神经生物学：

内田桂予子 ら: "Induction of apoptosis by phosphotidylserine" J.Biol.Chem.(印刷中). (1998)

Keiko Uchida 等人：“磷脂酰丝氨酸诱导细胞凋亡”J.Biol.Chem.（出版中）。

K.Hanada, et al.: "Mammalian cell mutants resistant to a sphingomyelin-directed bytolysin"J.Biol.Chem.. 273. 33787-33794 (1998)

K.Hanada 等人：“对鞘磷脂引导的溶菌素具有抗性的哺乳动物细胞突变体”J.Biol.Chem.. 273. 33787-33794 (1998)