Membrane phospholipid dynamics and cytoskeletal organization: Regulation of actin filament assembly by redistribution of membrane surface phospholipid

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Using a novel peptide probe, Ro09-0198, which specifically forms a tight equimolar complex with phosphatidylethanoalmaine (PE) on biological membranes, we have analyzed the cell surface movement of PE in dividing CHO cells. We found that flip-flop of plasma membrane PE was markedly enhanced at the cleavage furrow during the late telophase of cytokinesis and that cell surface trapping of PE induced by adding the peptide-streptavidin complex to the mitotic cells blocked cytokinesis at the late telophase. Further analysis have shown that the redistribution of plasma membrane phospholipids acts as a regulator of actin filament assembly and play a crucial role in mediating a coordinate movement between plasma membrane and cytoskeleton. Molecules involved in the cross-talk between the phospholipid dynamics and cytoskeletal reorganization have been investigated using yeast and CHO mutant cells which were selected by their altered sensitivity to the PE-binding peptide.