Phospholipid translocase : Its role in cytoskeletal reorganization.

磷脂转位酶：其在细胞骨架重组中的作用。

• 批准号: 07680788
• 负责人: UMEDA Masato
• 金额: $ 1.41万
• 依托单位: The Tokyo Metropolitan Institute of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680788/
• 关键词: phospholipid asymmetry; phospholipid translocase; flip-flop; anti-idiotypic antibody; cell division; cytokinesis; actin; cytoskeleton; ホスフロチジルエタノールアミン; 膜融合; ホスファチジルセリン; ホスファチジルエタノールアミン

Recent advances in molecular genetics have provided powerful tools for studying the biological function of proteins, resulting in rapid extension of our knowledge of their functions. However, little is still known about the organization and function of biological membranes, especially how membrane phospholipids are organized and participate in cellular functions. This is mainly because of the lack of appropriate methodologies for either manipulating or tracing the function of membrane lipids. We have developed various probes to analyze the moleculaer motion and function of phospholipids in biological membranes. Our studies have shown that enhanced transbilayr movement of plasma membrane phospholipids plays a pivotal role in mediating coordinated movement between cytoskeletal proteins and plasma membrance to achieve successful cell dividion.

分子遗传学的最新进展为研究蛋白质的生物学功能提供了强有力的工具，使我们对蛋白质功能的认识迅速扩展。然而，关于生物膜的组织和功能，特别是膜磷脂是如何组织和参与细胞功能的，目前还知之甚少。这主要是因为缺乏适当的方法来操纵或追踪膜脂的功能。我们已经开发了各种探针来分析生物膜中磷脂的分子运动和功能。我们的研究表明，增强的质膜磷脂跨膜运动在细胞骨架蛋白和质膜之间的协调运动中起着关键作用，从而实现成功的细胞分裂。

项目成果

Tokita, S., Arai, M., Yamamoto, N., Katagiri, Y., Tanoue, K., Igarashi, K., Umeda, M., Inoue, K.: "Specific cross-reaction of IgG anti-phospholiopid antibody with platelet glycoprotein IIIa." Thrombosis. Haemostasis.75. 168-174 (1996)

Tokita, S.、Arai, M.、Yamamoto, N.、Katagiri, Y.、Tanoue, K.、Igarashi, K.、Umeda, M.、Inoue, K.：“IgG 抗磷脂的特异性交叉反应

五十嵐浩二ら: "Specific binding of a synthetic peptide derived from an antibody complementarity determining region to phoshatidylserine." J.Biol.Chem.117. 452-457 (1995)

Koji Igarashi 等人：“源自抗体互补决定区的合成肽与磷脂酰丝氨酸的特异性结合。”J.Biol.Chem.117 (1995)。

Igarashi, K., Asai, K., Kaneda, M., Umeda, M., Inoue, K.: "Specific binding of a synthetic peptide derived from an antibody complementarity determining region to phosphatidylserine." J.Biochem.117. 452-457 (1995)

Igarashi, K.、Asai, K.、Kaneda, M.、Umeda, M.、Inoue, K.：“源自抗体互补决定区的合成肽与磷脂酰丝氨酸的特异性结合。”

梅田真郷: "タンパク質の分子間相互作用実験法" 羊土社, 8 (1976)

Shingo Umeda：“蛋白质分子相互作用的实验方法”Yodosha，8（1976）

榎本和生ら: "Exposure of phosphatidy lethanolamine on the surfuce of apoptotic cells." Expertmental Cell Research. 232(印刷中). (1997)

Kazuo Enomoto 等人：“凋亡细胞表面的磷脂酰乙醇胺的暴露。”Expertmental Cell Research 232（出版中）。