Establishment and application of the strategies for the discovery of biomarker proteins and peptides including species bound to albumin in serum/plasma

我们建立了一种差示溶解(DS)法来提取小分子蛋白质/肽，该方法含有许多与血清中白蛋白等载体蛋白结合的多肽，具有良好的重复性和收率。使用DS方法，我们对1μL血清样本中的1,500多个多肽进行了高质量的比较分析。此外，我们对DS方法进行了优化，以选择性地浓缩小于6 kDa的多肽。将改进的DS法与LC-MS联用，发现了两个肾癌候选多肽。

We developed a differential solubilization (DS) method to extract low-molecular-weight proteins/peptides containing many peptides bound to carrier proteins such as albumin in serum with good reproducibility and yield. Using the DS method we performed high-quality comparative analyses of more than 1500 peptides from 1μL of serum samples. Furthermore, we optimized the DS method to selectively enrich peptides less than 6 kDa. The modified DS method was combined with LC-MS and Two candidate peptides of renal cell carcinoma were discovered.