Development of safe and clean low-temperature sterilization usingwater vapor plasma

本研究的目的是制备稳定的H_2O等离子体，并对其灭菌效果和生物材料相容性进行评价。结果表明：（1）通过优化供气和排气的定时以及应用超低容量质量流量控制器（0.02g/min），可以稳定地产生H_2O等离子体。2)H_2O等离子体杀菌效果较好，可杀灭10^6G.（3）H_2O等离子体处理的多孔金属盘清除标准细胞相容性。

The aim of this study was to generate stable H_2O plasma and to evaluate its efficacy of sterilization and biomaterial compatibility. As the results of that, 1) stable generation of H_2O plasma was possible by optimization the timing of air supply and an exhaust gas and application of ultra low-volume mass flow controller (0.02 g/min). 2) H_2O plasma showed high sterilization effect to kill 10^6 G. stearothermophilus spores wrapped with tyvec^R at 60oC in 10 min. 3) porus metal disc treated with H_2O plasma cleared standard cell compatibility.