Study on the stemness of human dental pulp cells and the inductive efficiency for the safty iPS cells

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In this study, we examined the effects of hypoxia and cell stemness on the inductive efficiency for the safty iPS cell using human dental pulp cells. Our results were as follows; 1. Hypoxia condition(3%O2) improved the cell growth and establishment efficiency of primary culture. Furthermore, hypoxia condition was effective and useful for the maintenance of stro-1positive cells and high differential potency cells and we found out that the hypoxia condition could improve the efficiency of iPS cell induction and its quality. 2. We established the iPS cells efficiently using episomal plasmid as a gene-transferring vector without the foreign gene insertion. 3. We found out that the DLX4 might play an important role in the cell initiation and that the enhancement of the DLX4 expression could induce the highly efficient effect for the iPS cell establishment.