Effects of betel quid chewing on the DNA methylation status of oral mucosa in south Asia

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To reveal the effects of DNA methylation status on the oral cancer carcinogenesis, we carried out the analysis of lesions in in betel quid chewers. First of all, we evaluated the swab sample accuracy using swab and tissue samples in promoter region of p16 and MGMT so on. DNA methylation status was well correlated between swab and tissue samples. Then, we analyzed DNA methylation status of Taiwan swab samples using pyrosequencing method focused on p16 and MGMT. 10% samples indicated positive. Furthermore, we examined the methylation positive of p16 in cancer patients and detected it in high ratio (almost 100%). These results possibly suggested the usefulness of p16 analysis as a biomarker in oral cancer.