Infection dynamics and molecular mechanisms of prevalence of new variant and emerging Rotaviruses prevailing in Asia

亚洲流行的新变种和新出现的轮状病毒的感染动态和分子机制

• 批准号: 22406017
• 负责人: KOBAYASHI Nobumichi
• 金额: $ 12.48万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22406017/
• 关键词: ロタウイルス; 遺伝子型; 新変異型; 新興型; 分子疫学; アジア; 系統樹; 遺伝子再集合(リアソートメント); 遺伝子再集合体; 全遺伝子分節; 遺伝子配列; 系統解析; 変異型; リアソートメント; 系統; ゲノム; 全ゲノム

To elucidate genetic characteristics of new variants and emerging types of rotavirus that spread to mainly in Asian countries, whole genomic sequence analysis and phylogenetic analysis were conducted. In China and Bangladesh, the predominant genotypes were G1P[8]/G3P[8], G2P[4], respectively, among which reassortant viruses containing gene segments derived from animal rotavirus were detected. Rotaviruses with P[8]b, a new variant of P[8]-VP4 genotype, were detected in China, Bangladesh, and Myanmar, and mostly associated with globally emerging type G9. Whole genomic analysis of various rotaviruses strains with emerging genotypes from Asia and other regions (genotypes G1P[6], G2P[6], G3P[2]、G3P[6], G3P[9], G4P[10], G6P[9], G8P[1], G9P[19]) revealed that these strains could be classified into 1)reassortant between different human rotavirus genogroups, 2)reassortant between human and animal rotaviruses, and 3) animal rotaviruses that directly transmitted to humans. These findings indicated that reassortment and interspecies transmission may be the main causing mechanisms of new variants and emergingtypes of human rotaviruses.

为了阐明主要在亚洲国家传播的轮状病毒的新变体和新类型的遗传特征，进行了全基因组序列分析和系统发育分析。在中国和孟加拉国，主要基因型分别为G1 P [8]/G3 P [8]和G2 P [4]，其中也检出了含有动物源轮状病毒基因片段的回复病毒。P[8]B轮状病毒是P[8]-VP 4基因型的一种新变体，在中国、孟加拉国和缅甸检出，主要与全球新出现的G9型相关。亚洲和其他地区不同轮状病毒新基因型株的全基因组分析G1 P [6]、G2 P [6]、G3 P [2]、G3 P [6]、G3 P [9]、G4 P [10]、G6 P [9]、G8 P [1]、G9 P [19]基因型的分离结果表明，这些毒株可分为1）不同人轮状病毒基因组间的分离株，2）人和动物轮状病毒之间的分离株，3）直接传播给人类的动物轮状病毒。这些结果表明，重组和种间传播可能是新的变种和新出现的类型的人轮状病毒的主要机制。

项目成果

Ghosh S, Paul SK, Yamamoto D, Nagashima S, Kobayashi N.

Ghosh S、Paul SK、Yamamoto D、Nagashima S、Kobayashi N.

• 发表时间: 2011
• 期刊: Infect Genet Evol
• 影响因子: 3.2
• 作者: Ghosh S;Paul SK;Yamamoto D;Nagashima S;Kobayashi N.
• 通讯作者: Kobayashi N.

Analysis on genetic diversity and molecular evolution of human group B rotaviruses based on whole genome segments.

基于全基因组片段的人类B族轮状病毒遗传多样性和分子进化分析

• 发表时间: 2010
• 期刊: Journal of General Virology
• 影响因子: 3.8
• 作者: Yamamoto D.;et al.
• 通讯作者: et al.

Interspecies transmission of rotaviruses evidenced by whole genomic sequence analysis.

全基因组序列分析证明轮状病毒的种间传播。

• 发表时间: 2013
• 作者: Kobayashi N;Ghosh S
• 通讯作者: Ghosh S

Interspecies transmission of rotaviruses evidenced by whole genomic sequence analysis

全基因组序列分析证明轮状病毒的种间传播

• 发表时间: 2013
• 作者: Kobayashi N;Ghosh S.
• 通讯作者: Ghosh S.

Whole genomic analysis of a rare human G1P[9] rotavirus strain.

罕见人类 G1P[9] 轮状病毒株的全基因组分析。

• 发表时间: 2012
• 作者: Ghosh S;Shintani T;Taniguchi K;Kobayashi N
• 通讯作者: Kobayashi N