Identification and molecular epidemiological analysis of emerging clones of community-acquired MRSA

社区获得性 MRSA 新兴克隆的鉴定和分子流行病学分析

• 批准号: 23590746
• 负责人: KOBAYASHI Nobumichi
• 金额: $ 3.49万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590746/
• 关键词: MRSA; 市中感染; 分子疫学; PVL; USA300; 国際研究者交流; バングラデシュ; ミャンマー

To investigate prevalence in Japan of community-acquired MRSA (CA-MRSA) which is concerned for its global spread, and their molecular epidemiological characteristics, we analyzed clinical isolates from the Sapporo Medical University Hospital (601 isolates, 2008-2010) and those from local hospitals/clinics in Hokkaido prefecture (outpatients, 422 isolates in 2011). As a result, SCCmec types IV and V, which are genetic markers of CA-MRSA, were detected in 4.3% and 17.1% in the isolates from the University Hospital and local hospitals/clinics, respectively. Among them, seven isolates were revealed to be USA300 clone which is dominant in the US (ST8, positive for PVL and ACME), and many ST5 MRSA having ACME were detected. These emerging CA-MRSA clones were confirmed to be distributed in Japan.

为了调查日本社区获得性MRSA（CA-MRSA）的流行情况及其分子流行病学特征，我们分析了来自札幌医科大学医院的临床分离株（601株分离株，2008-2010年）和来自北海道县当地医院/诊所的临床分离株（门诊患者，2011年422株分离株）。结果，在大学医院和当地医院/诊所的分离株中分别检测到4.3%和17.1%的SCCmec IV型和V型（CA-MRSA的遗传标记）。其中7株为USA 300克隆，为美国的优势株（ST 8，PVL和ACME阳性），并检出多株ST 5型ACME耐甲氧西林金黄色葡萄球菌。这些新出现的CA-MRSA克隆被证实分布在日本。

项目成果

Genetic diversities in ACME-SCCmec composite islands in CA-MRSA isolates collected in Hokkaido.

北海道收集的 CA-MRSA 分离株中 ACME-SCCmec 复合岛的遗传多样性。

• 发表时间: 2012
• 作者: 漆原範子;川口谷充代;小林宣道
• 通讯作者: 小林宣道

Two Novel Staphylococcal Cassette Chromosome mec Elements Containing Two Copies of mecA genes Identified in Bangladeshi Clinical Isolates of Staphylococcus haemolyticus.

在孟加拉国溶血葡萄球菌临床分离株中鉴定出含有两个 mecA 基因拷贝的两个新型葡萄球菌盒染色体 mec 元件。

• 发表时间: 2013
• 作者: Urushibara N.;Kawaguchiya M.;Paul S.K.;Kobayashi;N.
• 通讯作者: N.

Genetic analysis of PVL/ACME- positive Staphylococcus aureus isolated in a Japanese hospital

日本某医院分离的PVL/ACME阳性金黄色葡萄球菌的遗传分析

• 发表时间: 2011
• 作者: Kawaguchiya M;Urushibara N;Yamamoto D;Shinagawa M;Watanabe N;Kobayashi
• 通讯作者: Kobayashi

Characterization of two novel arginine catabolic mobile element (ACME) and staphylococcal chromosome cassette mec (SCCmec) composite islands found in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA).

社区获得性耐甲氧西林金黄色葡萄球菌 (CA-MRSA) 中发现的两种新型精氨酸分解代谢移动元件 (ACME) 和葡萄球菌染色体盒 mec (SCCmec) 复合岛的表征。

• 发表时间: 2011
• 作者: Urushibara N;Kawaguchiya M;Kobayashi N.
• 通讯作者: Kobayashi N.

Serotype distribution and analysis of macrolide- and penicillin-resistance among non-invasive S. pneumoniae isolates in northern Japan

日本北部非侵袭性肺炎链球菌血清型分布及大环内酯类和青霉素耐药性分析

• 发表时间: 2013
• 作者: Kawaguchiya M;Urushibara N;Ghosh S;Ito M;Kudo K;Kuwahara O;Kobayashi N
• 通讯作者: Kobayashi N