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Elucidation of prevalence of group B human rotavirus in Asia

阐明亚洲 B 族人类轮状病毒的流行情况

基本信息

批准号：
12576011
负责人：
KOBAYASHI Nobumichi
金额：
$ 9.79万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12576011/
关键词：
rotavirus group B adult diarrhea India Bangladesh China CAL-1 バングラディシュ

项目摘要

In the present study, group B rotaviruses were detected in India (9 strains in 4086 specimens), Bangladesh (14 strains in 1151 specimens), and China (2 strains in 769 specimens), mostly in specimens from sporadic diarrheal cases of adult patients. Genetic analysis was done for CAL-1 strin in India, Wh683 strain in China, and four strains (Bang373, Bang544, Bang334, and Bang402) in Bangladesh. The Chinese strain Wh683 which was obtained in Wuhan city in 2000 was genetically extremely closeer (sequence identity was 98-99%) to the ADRV strain (a prototype of human group B rotacirus detected in 1982) than to CAL-1 or Bang373 (identities of 90-95%), indicating that group B rotaviruses in China are highly conserved. In contrast, VP7 gene sequences of the Bangladeshi group B rotaviruses were almost identical each other and showed also high sequence identity to Indian strain CAL-1 (98%) and also to Chinese strain ADRV (92%), while identities to bovine and murine viruses were considerably low (60-63%). Other genes of Bang373 and Bang544 encoding VP2, VP4, VP6, and NSP1〜NSP5 showed also extremely high sequence identities to those of CAL-l (97.7-99.4%) than those of ADRV (89.9-93.9%). Characterization of nucleotide substitutions among Bang373, CAL-1, and ADRV suggested that all the gene segments might have evolved neutrally at similar mutaton rates. In conclusion, group B rotaviruses detected in Bangladesh represented by Bang373 and the Indian virus CAL-1 were considered as virtually identical viruses which are genetically distinct from ADRV, and it was suggested that Bang373 (CAL-l)-like group B rotavirus (Bengali strains) might have been primarily distributed to an are around the Bay of Bengal.

在本研究中，在印度(4086份标本中检出9株轮状病毒)、孟加拉国(1151份标本中检出14株轮状病毒)和中国(769份标本中检出2株B组轮状病毒)中检出B组轮状病毒，主要发生在散发性成人腹泻病例的标本中。对印度的CAL-1毒株、中国的WH683毒株和孟加拉国的4个毒株(邦373、邦544、邦334和邦402)进行了遗传分析。2000年在武汉市获得的中国轮状病毒株Wh683与1982年发现的人B群轮状病毒原型株(ADRV)的同源性为98-99%，而与CAL-1或邦373株的同源性为90-95%，说明中国的B组轮状病毒是高度保守的。孟加拉B组轮状病毒的VP7基因序列几乎完全相同，与印度株CAL-1和中国株ADRV也有很高的同源性(98%)和中国株(92%)，而与牛和鼠病毒的同源性很低(60%~63%)。Bang 373和Bang 544编码VP2、VP4、VP6和NSP1~NSP5的基因与CAL-L的同源性(97.7-99.4%)也高于ADRV的同源性(89.9-93.9%)。对Bang373、CAL-1和ADRV之间核苷酸替换的特征表明，所有基因片段可能以相似的突变率中性进化。总之，在孟加拉国检测到的以邦373为代表的B组轮状病毒与印度的CAL-1病毒被认为是在基因上不同于ADRV的几乎相同的病毒，这表明B组轮状病毒(孟加拉株)可能主要分布在孟加拉湾周围的一个区域。