Subtypes ofmicroglia/macrophages relevant to the diagnosis and treatment of brain tumors

与脑肿瘤诊断和治疗相关的小胶质细胞/巨噬细胞亚型

• 批准号: 22500321
• 负责人: SASAKI Atsushi
• 金额: $ 2.91万
• 依托单位: Saitama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500321/
• 关键词: ミクログリア; マクロファージ; 脳腫瘍; グリオーマ; トランスジェニックラット; v-erbB; TAM; Iba1; 上皮成長因子受容体

Glioma-infiltrating microglia/macrophages are referred to as tumor-associated macrophages (TAMs). Transgenic (TG) rats expressingv-erbB, which is a viral form of the epidermal growth factor receptor, under transcriptional regulation by the S100-βpromoter develop brain tumors. We carried out immunohistochemical and morphometrical analyses of microglia/macrophages both in human gliomas and in the experimental tumors. Based onthe results, we found that the Iba1-positive, TAM with the morphology of activated/phagocytic cells were consistently found within glioma tissues. Iba1-positive TAMs of tumor core were significantly more activated than Iba1-positive microglia of non-neoplastic brain tissue in intraparenchymal, anaplastic oligodendrogliomas. In contrast to human gliomas, most TAMs in the experimental gliomas showed no or little expression against CD68, CD163, or CD204, although CD204-positive TAMs were observed in necrosis as well as in proliferating vascular wall. In conclusion, S-100β-v-erbB TG rats may serve as a useful animal model for further analysis of TAMs in terms of tumor cell proliferation, microvascular proliferation and phagocytosis, and as a tool for therapeutic use in malignant gliomas, although it should be noted that the polarization of TAMs towards the M2 phenotype remains unclear.

胶质瘤浸润的小胶质细胞/巨噬细胞被称为肿瘤相关巨噬细胞（TAM）。表达v-erbB（一种表皮生长因子受体的病毒形式）的转基因（TG）大鼠在S100-β启动子的转录调节下发展成脑肿瘤。我们对人类神经胶质瘤和实验肿瘤中的小胶质细胞/巨噬细胞进行了免疫组织化学和形态计量学分析。基于结果，我们发现在胶质瘤组织中一致发现Iba1阳性、具有活化/吞噬细胞形态的TAM。在实质内间变性少突胶质细胞瘤中，肿瘤核心的 Iba1 阳性 TAM 明显比非肿瘤性脑组织的 Iba1 阳性小胶质细胞更活跃。与人类神经胶质瘤相反，尽管在坏死和增殖的血管壁中观察到 CD204 阳性 TAM，但实验神经胶质瘤中的大多数 TAM 没有或很少表现出针对 CD68、CD163 或 CD204 的表达。总之，S-100β-v-erbB TG 大鼠可作为进一步分析 TAM 在肿瘤细胞增殖、微血管增殖和吞噬作用方面的有用动物模型，并作为恶性胶质瘤治疗用途的工具，但值得注意的是 TAM 向 M2 表型的极化仍不清楚。

项目成果

脳腫瘍の治療に有益な病理診断をめざして

旨在对脑肿瘤治疗有用的病理诊断

• 发表时间: 2010
• 作者: 三島一彦;佐々木惇;鈴木智成;脇谷健司;安達淳一;小林正人;藤巻高光;西川亮;中里洋一
• 通讯作者: 中里洋一

新時代の脳腫瘍学 診断・治療の最前線 膠芽腫、星細胞腫

脑肿瘤新时代：胶质母细胞瘤、星形细胞瘤诊治前沿

• 发表时间: 2010
• 作者: Ito H;Nakayama K;Jin C;Suzuki Y;Yazawa I.;佐々木惇
• 通讯作者: 佐々木惇

新時代の脳腫瘍学-診断・治療の最前線

脑肿瘤新时代——诊疗最前沿

• 发表时间: 2010
• 作者: Ishida A;Shibuya M;Komori T;et al;佐々木惇
• 通讯作者: 佐々木惇

病理と臨床臨時増刊号病理形態学キーワード

病理学和临床特刊病理形态学关键词

• 发表时间: 2010
• 作者: Yazawa;I.;横尾英明
• 通讯作者: 横尾英明

Central Nervous System Lymphoma Initially Diagnosed as Tumefactive Multiple Sclerosis after Brain Biopsy

• DOI: 10.2169/internalmedicine.52.8531
• 发表时间: 2013-01-01
• 期刊: INTERNAL MEDICINE
• 影响因子: 1.2
• 作者: Ohe, Yasuko;Hayashi, Takeshi;Tanahashi, Norio
• 通讯作者: Tanahashi, Norio