Development of HPLC separation system of bio-based highly hydrophilic compounds with ultrahigh separation efficiencies.

基于亲水性和对被分析物部分结构的选择性，建立了亲水作用色谱固定相的表征方法。采用自由基聚合法，建立了硅胶整体柱的功能化方法。通过控制聚合过程中单体浓度，可以提高高极性化合物的亲水保留率。与市售HPLC柱相比，实现了显著更高的亲水性和更高的分离效率。

Characterization method for hydrophilic interaction chromatographic stationary phases, based on hydrophilicity and selectivity for partial structure of analytes was developed. Using radical polymerization, functionalization method of monolithic silica columns was established. We could increase hydrophilic retention of highly polar compounds, by controlling monomer concentration of polymerization process. Significantly higher hydrophilicity and higher separation efficiency compared to commercially available HPLC columns were realized.