Development of comprehensive two-dimensional HPLC for high-performance separations in the future
Development of comprehensive two-dimensional HPLC for high-performance separations in the future
批准号:
14340234
负责人:
TANAKA Nobuo
金额:
$7.55万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2002
资助国家:
日本
项目状态:
已结题
起止时间:
2002 至 2004
中文摘要
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英文摘要
The objective of this research project was to increase peak capacity of HPLC by developing simple and comprehensive two dimensional (2D) HPLC. By employing a monolithic silica-C18 column at the second dimension (2nd-D), we were able to obtain the following results.(1) We proved the concept of simple and comprehensive 2D-HPLC in a reversed-phase-reversed-phase mode by using a particle-packed column of fluoroalkyl-bonded silica as a first-dimension (1st-D) column, and C18- or pentabromobenzyloxypropyl-bonded monolithic silica as a 2nd-D column.(2) By utilizing the difference in selectivity provided by organic solvents, 2D-HPLC was carried out using monolithic silica C18 columns both at 1st-D and at 2nd-D.(3) 2D-HPLC was carried out by using an ion-exchange column packed with polymer particles at 1st-D and a monolithic silica-C18 column or a monolithic silica capillary column at 2nd-D for peptide separations.The use of these systems produced (1)peak capacity of up to 1100 in methanol-water mobile phase, (2)peak capacity of 700 or greater for hydrocarbons and benzene derivatives, respectively. The ion-exchange-reversed-phase 2D-HPLC system provided (3)peak capacity of 700 or greater for BSA digest with a short monolithic silica column at 2nd-D, and peak capacity of about 500 for BSA digest by using a 0.2 mmlD, 10 cm long capillary column at 2nd-D, respectively. Optimization of 2D-HPLC conditions were attempted to show the possibility of achieving peak capacity of greater than 2000 in one hour. The flow rate, retention factors of solutes, and column length at 2nd-D were shown to dominate the peak capacity of a 2D-HPLC system. Monolithic silica columns were found to be best suited for 2D-HPLC based on their high permeability and high performance at high linear velocity of mobile phase.
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DOI:
10.1002/rcm.1376
发表时间:
2004-01-01
期刊:
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
影响因子:
2
作者:
[Wienkoop, S, Glinski, M, Weckwerth, W]
通讯作者:
Weckwerth, W
M.Turowski, et al.: "Deuterium Isotope Effects on Hydrophobic Interactions. The Importance of Dispersion Interactions in the Hydrophobic Phase"J.Am.Chem.Soc.. 125. 13836-13849 (2003)
M.Turowski 等:“氘同位素对疏水相互作用的影响。疏水相中分散相互作用的重要性”J.Am.Chem.Soc.. 125. 13836-13849 (2003)
DOI:
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影响因子:
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作者:
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通讯作者:
N.Tanaka, H.Kobayashi: ""Monolithic Silica Columns For Capillary liquid cromatography" in "Monolithic Columns""Elsevier(印刷中). (2002)
N.Tanaka、H.Kobayashi:“Monolithic Columns”中的“毛细管液相色谱用整体硅胶柱”(Elsevier)(2002 年出版)。
DOI:
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作者:
[]
通讯作者:
Capillary electrochromatography on monolithic silica columns.
整体硅胶柱上的毛细管电色谱法。
DOI:
--
发表时间:
2002
期刊:
Anal.Sci. 18
影响因子:
--
作者:
[H.Kobayashi, C.Smith, K.Hosoya, T.Ikegami, N.Tanaka]
通讯作者:
N.Tanaka
N.Tanaka, et al.: "Monolithic Silica Columns For High-Efficiency Chromatographic Separations"Journal of Chromatography, A. 965. 35-49 (2002)
N.Tanaka 等:“用于高效色谱分离的整体硅胶柱”色谱杂志,A. 965. 35-49 (2002)
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发表时间:
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作者:
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通讯作者:
共 32 条
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Essential control of sound and vibration in a strongly-coupled system
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财政年份:2009
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Development of high-speed and high-efficiency monolithic silica capillary columns for high-performance liquid chromatography
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批准号:20350036
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项目类别:Grant-in-Aid for Scientific Research (B)
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UHV in-situ and Cs-corrected HR(S)TEM Studies of structures and growth process of Ge nanodots formed on faintly oxidized Si surfaces
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Generation of quieter infrastructure for nanometer technology
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Study of structural fluctuation of nanomaterials by Cs-corrected electron lenses
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批准号:17201022
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$31.28万
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Active control of smart structure using cluster control
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批准号:15360124
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.28万
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财政年份:2003
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Milli-second time-resolved HRTEM of absorption / desorption of hydrogen
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.64万
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财政年份:2001
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依托单位:
Development of monolithic silica columns in capillary for high-speed and high-performance HPLC
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批准号:12554032
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项目类别:Grant-in-Aid for Scientific Research (B)
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Adaptation and change of immune function to stresses of heat and exercise
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财政年份:1999
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Development of porous silica rod columns for higher performance of HPLC
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资助金额:$8.64万
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财政年份:1997
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Chromatographic techniques for PCB and dioxin problems.
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批准号:09044081
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项目类别:Grant-in-Aid for Scientific Research (B).
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财政年份:1997
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An approach to the extreme in separation science : recognition of isotopic chirality
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Chromatographic Approach to Environmental Science of Dioxins
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批准号:08044079
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$1.79万
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财政年份:1996
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负责人:TANAKA Nobuo
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依托单位:
Study of semiconductor/metal interfaces by coherent nano-beam electron diffraction.
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批准号:07455023
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$3.97万
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财政年份:1995
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负责人:TANAKA Nobuo
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Development of RHEED apparatus using spin-polarized electron source for the study of surface and interface magnetism.
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批准号:07555008
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$3.71万
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财政年份:1995
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负责人:TANAKA Nobuo
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Chromatographic Approach to Environmental Sciences of Dioxin
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批准号:07044084
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$1.92万
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财政年份:1995
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负责人:TANAKA Nobuo
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Detailed structure-analysis by using electron probe
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批准号:06302022
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资助金额:$7.23万
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负责人:TANAKA Nobuo
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海外基金