Development of highly efficient separation methods of saccharides using hydrophilic interaction liquid chromatography

在直径100-200 mm的熔融硅毛细管中，通过甲基丙烯酸酯的柱上自由基聚合，使具有各种亲水/离子交换功能的整体硅柱功能化。这些具有亲水性、弱阳离子交换和弱阴离子交换功能的色谱柱，可用于单糖和低聚糖及其衍生物的分离，以评价其分离效率。在糖、核苷和核苷酸的分离中，在高效液相系统的板数和分离速度方面，它们可以提供比商业颗粒填充柱更高的分离效率。

Monolithic silica columns in fused silica capillaries of 100-200mm diameters were functionalized by on-column radical polymerization of methacrylates possessing various hydrophilic/ion-exchange functions. These columns with hydrophilic, weak cation exchange, and weak anion exchange functions, were provided for the separation of mono- and oligosaccharides and their derivatives using microHPLC systems to evaluate their separation efficiencies. They could provide higher separation efficiencies than commercial particle-packed columns in terms of plate numbers of the HPLC system, and separation speed, in the separation of saccharides, nucleosides, and nucleotides.