Optical enzyme assay with a fluorogenic liposome

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Cationic polymer-amphiphilic anion complexes can easily cross the lipid bilayer membrane, which can be detected as fluorescence emission by using a fluorogenic liposome. Presence of competitive anions that hinder the formation of polymer-counteranion complexes should reduce the translocation activity. Consumption of the competitive anions or production of activator anions during chemical reactions led into fluorogenic response. Activities of enzymes, such as protein kinase and acetylcholine esterase, and their inhibitors were readily evaluated by the “naked eye” detection system. In addition, we successfully estimated the flavor concentrations of lactate in sour milk, lipid in cow’s milk. The simple mix-and-measure method will be a cost-effective multi-component flavor sensor.