High-through put isolation of functional mutants of actin using Dictyostelium expression system

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Actin filaments are found in all eukaryotic cells and are essentialfor many of their movements including cell migration and cell division. We found that the cell behavior of Dictyostelium cells changed, when the vector for the expression of mutant actin was incorporated. Using this phenomenon, functional mutants of actin were isolated efficiently. It was found that the tyrosine-143 of actin is important for actin assembly and the activation of myosin ATPase. The crystal structures of such mutants were determined.