Mechanism of primitive polarity formation in epithelial cells independent of cell-cell contact

上皮细胞中原始极性形成的机制与细胞-细胞接触无关

• 批准号: 22570195
• 负责人: YONEMURA Shigenobu
• 金额: $ 3万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570195/
• 关键词: 上皮細胞; 極性; ミオシン; 細胞外基質; 上皮極性; 微小管; 細胞周期; 細胞極性; 細胞間接着装置; 細胞接着装置

I aimed elucidation of the molecular mechanism of polarity formation of epithelial cells. By knocking down a number of genes known to be involved in polarity formation or in force generation through actomyosin interaction, I confirmed the roles of the known factors. Furthermore, I found that myosin motor activity is important for positioning of the cell-cell junctions according to the polarity. Moreover, positional cues from the ECM are not required for establishment of the epithelial polarity but important for alignment of the polarity of each cell.

旨在阐明上皮细胞极性形成的分子机制。通过敲除一些已知参与极性形成或通过肌动球蛋白相互作用产生力的基因，我证实了已知因素的作用。此外，我发现肌球蛋白运动活性对于根据极性定位细胞-细胞连接是重要的。此外，来自ECM的位置线索不是建立上皮极性所必需的，但对于每个细胞的极性排列很重要。

项目成果

隣り合う細胞を引っ張る力が多細胞形態形成を制御する

邻近细胞的拉力控制多细胞形态发生

• 发表时间: 2012
• 作者: Yonemura;S;米村重信;米村重信
• 通讯作者: 米村重信

Hippo pathway regulation by cell morphology and stress fibers

• DOI: 10.1242/dev.070987
• 发表时间: 2011-09-15
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Wada, Ken-Ichi;Itoga, Kazuyoshi;Sasaki, Hiroshi
• 通讯作者: Sasaki, Hiroshi

Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cells.

• DOI: 10.1083/jcb.201106101
• 发表时间: 2012-04-30
• 期刊: The Journal of cell biology
• 作者: Inoko A;Matsuyama M;Goto H;Ohmuro-Matsuyama Y;Hayashi Y;Enomoto M;Ibi M;Urano T;Yonemura S;Kiyono T;Izawa I;Inagaki M
• 通讯作者: Inagaki M

Furuse M. Snakeskin, a membrane protein associated with smooth septate junctions, is required for intestinal barrier function in Drosophila

Furuse M. Snakeskin 是一种与平滑隔膜连接相关的膜蛋白，是果蝇肠道屏障功能所必需的

• 发表时间: 2012
• 期刊: J Cell Sci.
• 作者: Yanagihashi Y;Usui T;Izumi Y;Yonemura S;Sumida M;Tsukita S;Uemura T
• 通讯作者: Uemura T

Mechanosensing function of alpha-catenin in epithelial morphogenesis

α-连环蛋白在上皮形态发生中的机械传感功能

• 作者: 米村重信;柴田真依;米村重信;Yonemura S
• 通讯作者: Yonemura S