Detections of genes and proteins related proanthocyanidins synthesis from catechins

儿茶素合成原花青素相关基因和蛋白质的检测

• 批准号: 22580011
• 负责人: MATSUI Katsuhiro
• 金额: $ 2.5万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580011/
• 关键词: 植物育種; 遺伝; プロアントシアニジン; MYB転写因子; CRES-T; 二次代謝産物; 形質転換体; フラボノイド; トランジエントアッセイ; 自殖性ソバ; 転写因子; MYB; 分離集団

In this study, I had clarified that a gene of MYB transcription factor which was isolated from buckwheat was related to PA synthesis and regulations by production of transgenic plants. I also tried to detect genes and proteins relating synthesis PA by comparison of wild type and transgenic plants with cDNA-AFLP. I didn't find any specific genes by the way, and now I am preparing to do gel-free proteomic analysis to find them.

本研究通过转基因植物的生产，阐明了从荞麦中分离到的一个MYB转录因子基因与PA的合成和调控有关。本研究还利用cDNA-AFLP技术对转基因植株和野生型植株进行了PA合成相关基因和蛋白的检测。顺便说一下，我没有找到任何特定的基因，现在我正准备做无凝胶蛋白质组学分析来找到它们。

项目成果

QTL analysis of photoperiod sensitivity in common buckwheat by using markers for expressed sequence tags and photoperiod-sensitivity candidate genes.

• DOI: 10.1270/jsbbs.61.394
• 发表时间: 2011-12
• 期刊: Breeding science
• 影响因子: 2.4
• 作者: Hara T;Iwata H;Okuno K;Matsui K;Ohsawa R
• 通讯作者: Ohsawa R

Apple R2R3 MYB transcription factor, MdMYB2, acts as a repressor of anthocyanin biosynthesis

苹果 R2R3 MYB 转录因子 MdMYB2 充当花青素生物合成的抑制因子

• 发表时间: 2012
• 作者: Matsui K;Takos;A.M.;Parker J.L.;Robinson;S.P. and Walker;A.R.
• 通讯作者: A.R.