QTL analysis of photoperiod sensitivity in common buckwheat by using markers for expressed sequence tags and photoperiod-sensitivity candidate genes.
QTL analysis of photoperiod sensitivity in common buckwheat by using markers for expressed sequence tags and photoperiod-sensitivity candidate genes.
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DOI：
10.1270/jsbbs.61.394
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发表时间：
2011-12
期刊：
影响因子：
2.4
通讯作者：
Ohsawa R
中科院分区：
文献类型：
--
作者：
Hara T;Iwata H;Okuno K;Matsui K;Ohsawa R
光周期敏感性是荞麦的一个重要性状，与作物适应性和生态育种密切相关。虽然光周期敏感性被认为是由数量性状位点（QTL）控制的，但迄今为止还没有发现与光周期敏感性相关的基因或区域。本研究以02 AL 113（Kyukei SC2）LH.self和C 0408 -0 RP为母本，利用F4分离群体（n = 100），通过QTL分析，确定了控制光周期敏感性的QTL。利用与拟南芥光敏基因同源的3个标记和76个表达序列标签标记对F4代进行了基因分型。在荞麦中发现的3个光周期敏感性候选基因（FeCCA 1、FeELF 3和FeCOL 3）中，FeELF 3与光周期敏感性相关。Fest_L0606_4和Fest_L0337_6两个EST区域与光周期敏感性相关，分别解释了20.0%和14.2%的表型变异。对于这两个EST区域，来自02 AL 113（Kyukei SC2）LH.自身的等位基因导致早花。还证实了Fest_L0606_4和Fest_L0337_6之间的上位相互作用。这些结果表明，普通荞麦光周期敏感性是由光周期敏感性候选基因以及多基因作用组成的途径控制的。
Photoperiod sensitivity is an important trait related to crop adaptation and ecological breeding in common buckwheat (Fagopyrum esculentum Moench). Although photoperiod sensitivity in this species is thought to be controlled by quantitative trait loci (QTLs), no genes or regions related to photoperiod sensitivity had been identified until now. Here, we identified QTLs controlling photoperiod sensitivity by QTL analysis in a segregating F4 population (n = 100) derived from a cross of two autogamous lines, 02AL113(Kyukei SC2)LH.self and C0408-0 RP. The F4 progenies were genotyped with three markers for photoperiod-sensitivity candidate genes, which were identified based on homology to photoperiod-sensitivity genes in Arabidopsis and 76 expressed sequence tag markers. Among the three photoperiod-sensitivity candidate genes (FeCCA1, FeELF3 and FeCOL3) identified in common buckwheat, FeELF3 was associated with photoperiod sensitivity. Two EST regions, Fest_L0606_4 and Fest_L0337_6, were associated with photoperiod sensitivity and explained 20.0% and 14.2% of the phenotypic variation, respectively. For both EST regions, the allele from 02AL113(Kyukei SC2)LH.self led to early flowering. An epistatic interaction was also confirmed between Fest_L0606_4 and Fest_L0337_6. These results demonstrate that photoperiod sensitivity in common buckwheat is controlled by a pathway consisting of photoperiod-sensitivity candidate genes as well as multiple gene action.
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影响因子：
64.8
作者：
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通讯作者：
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