Distribution of lignin precursors in tree cells

采用基质辅助激光解吸/电离成像质谱法（MALDI-IMS）对几种日本软木木质部的单酚类糖苷进行了鉴定。MALI-IMS联合四氧化锇气相处理，使我们能够区分具有相同分子量的蔗糖和松柏素的分布。利用MALDI-IMS对日本柏木正常木材的横截面进行分析，发现针叶素在次生壁形成过程中主要分布在管胞中。

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) was applied to detect monolignol glucosides in differentiating xylems of several Japanese softwoods. MALI-IMS combined with osmium tetroxide vapor treatment enabled us to distinguish distribution of sucrose and coniferin with the same molecular weight. MALDI-IMS using transverse sections of normal wood in Japanese cypress (Chamaecyparis obtusa) showed that coniferin was mainly distributed in tracheids during secondary wall formation.