The functional changes of xylem parenchyma cells with respect to cell age of parenchyma cells analyzed by cryo-TOF-SIMS

本研究的目的是阐明活树木质部薄壁细胞的功能。在这个项目中，我们分析了化学物质积累的性质，细胞水平相对于薄壁细胞的细胞年龄。利用低温飞行时间二次离子质谱仪（cryo-TOF-西姆斯）和激光显微切割与气相色谱相结合的半定量和定性分析方法，分析了不同年龄的细胞的化学性质，结果表明，不同年龄的细胞化学性质存在差异，边材、中间材和心材的化学性质有明显区别。

The goal of this study is to clarify the function of xylem parenchyma cells in living trees. In this project, we analyzed the properties of chemical accumulation by cellular level with respect to cell age of parenchyma cells. The properties were analyzed by the cryo-TOF-SIMS which was able to visualize chemical distributions in a frozen sample, and semi-quantity and qualitative analysis which was combination of laser micro dissection with gas chromatography etc. As a result, the properties were different among cells in same age, while it was clearly distinguished between sapwood, intermediate wood, and heartwood.