Mechanism of gene expression regulation of anti-oxidant enzymeswith oxidative stress in fishes - Molecular cell biological approach -
Mechanism of gene expression regulation of anti-oxidant enzymeswith oxidative stress in fishes - Molecular cell biological approach -
批准号:
22580224
负责人:
OSATOMI Kiyoshi
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012
中文摘要
在这项研究中,我们检测了迟缓爱德华氏菌(E.tarda)菌株胞外产物对巨噬细胞NO和肿瘤坏死因子-α的诱导作用。迟缓艾美耳球虫强毒株(NUF251)的胞外蛋白以浓度依赖的方式刺激巨噬细胞产生NO和肿瘤坏死因子-α。对迟发性E.tarda菌株ECP的蛋白质组学分析表明,NUF251-ECP中主要成分为NUF251-ECP,与鞭毛蛋白序列具有同源性。
英文摘要
In this study, we examined the effects of extracellular products(ECP) from Edwardsiella tarda (E.tarda) strains on macrophages in terms of the induction of NO and TNF-α. ECP from virulent strain (NUF251) of E.tarda stimulated macrophages to induce NO and TNF-αproductions in a concentration-dependent manner. Proteomic analysis of ECP from E.tarda strains suggested that NUF251-specific protein, which has sequence homology with flagellin, is present in NUF251-ECP as a main component.
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DOI:
10.1016/j.cbpb.2010.12.007
发表时间:
2011-04
期刊:
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
影响因子:
--
作者:
[Yajun Wang;K. Osatomi;Y. Nagatomo;Asami Yoshida;K. Hara]
通讯作者:
Yajun Wang;K. Osatomi;Y. Nagatomo;Asami Yoshida;K. Hara
Purification, molecular cloning, and some properties of a manganese-containing superoxide disumutase from Japanese flounder (Paralichthys olivaceus)
日本牙鲆(Paralichthys olivaceus)含锰超氧化物歧化酶的纯化、分子克隆和一些特性
DOI:
--
发表时间:
2011
期刊:
Comparative Biochemistry and Physiology, Part B
影响因子:
--
作者:
[Y. Wang, K. Osatomi, Y. Nagatomo, A. Yoshida, K. Hara., 山下秀幸,東海 正, 石田祐三郎・杉田治男, 山下秀幸・堀川博史・東海正, Yajun Wang]
通讯作者:
Yajun Wang
Extracellular products from virulent strain of Edwardsiella tarda stimulate mouse macrophages (RAW264.7) to produce nitric oxide (NO) and tumor necrosis factor (TNF)-α.
迟缓爱德华氏菌强毒株的细胞外产物刺激小鼠巨噬细胞 (RAW264.7) 产生一氧化氮 (NO) 和肿瘤坏死因子 (TNF)-α。
DOI:
--
发表时间:
2010
期刊:
Fish&Shellfish Immunology
影响因子:
--
作者:
[Y. Wang, K. Osatomi, A. Yoshida, X. Liang, K. Kanai, T. Oda, K. Hara]
通讯作者:
K. Hara
Studies on structure, function and pathological significance of antioxidant enzymes in fishes.
鱼类抗氧化酶的结构、功能及病理意义研究。
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[山下秀幸・堀川博史・酒井猛, 他, 柳本 卓,山下秀幸,酒井 猛,明神寿彦,小林敬典, K. Osatomi]
通讯作者:
K. Osatomi
Edwardsiella tarda菌体外産生物質暴露に伴うマクロファージ系細胞株の応答
巨噬细胞系对迟发爱德华氏菌胞外物质暴露的反应
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[柳本卓・山下秀幸・酒井猛, 他, 王 亜軍]
通讯作者:
王 亜軍
Elucidation of the mechanism of evasion of the host immune response by the causative bacteria of Edwardsiellosis in fishes - From the viewpoint of reactive oxygen generation system -
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批准号:20K06228
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.75万
-
财政年份:2020
-
负责人:OSATOMI Kiyoshi
-
依托单位:
Molecular mechanism of the oxidative stress response caused by the anti-oxidant enzyme in fish - Molecular biological approach -
-
批准号:17K07940
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.08万
-
财政年份:2017
-
负责人:OSATOMI Kiyoshi
-
依托单位:
Molecular mechanism of the defense systems against host immunity by causative bacillus of Edwardsiellosis in fishes - Cell biological approach -
-
批准号:25450304
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.33万
-
财政年份:2013
-
负责人:OSATOMI Kiyoshi
-
依托单位:
Response mechanism of anti-oxidant enzymes with bacterial infection in fishes-Cell biological approach -
-
批准号:19580236
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.91万
-
财政年份:2007
-
负责人:OSATOMI Kiyoshi
-
依托单位:
Studies on superoxide dismutase and oxidative stress response in fishes
-
批准号:12660186
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.18万
-
财政年份:2000
-
负责人:OSATOMI Kiyoshi
-
依托单位:
海外基金