Analysis of amyloid fibril formation mechanism and cell toxicity of lysozyme

通过对核心肽的分析，证实溶菌酶的W62是淀粉样蛋白纤维形成的关键氨基酸。推测溶菌酶的纤颤可能是通过暴露在含有W62的区域的分子表面而发生的，导致主要由W62引起的分子缔合，形成排列的结构。溶菌酶原纤维的细胞毒性表现为细胞死亡，呈坏死状。另一方面，在人突变体在细胞内表达的情况下，细胞会导致分泌功能受损，导致溶菌酶在细胞内以不溶部分的形式积累。

It was confirmed that W62 of lysozyme is a crucial amino acid on amyloid fibrils formation by analysis of core peptide It is speculated that the fibrillation of lysozyme would occur by exposure to molecular surface of the region containing the W62, resulting in causing molecular association by mainly W62, and being formed the aligned structure. The cytotoxicity of lysozyme fibrils showed the cell death like necrosis. On the other hand, on the case of expression intracellular of the human mutant, cells cause impaired secretion, as a result, lysozyme cloud be accumulated as an insoluble fraction in cells.