In vitro and in vivo analyses of differentiation/proliferation mechanism of the Theileria orientalis protozoan parasite

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Theileria orientalis（T.o）parasite infection cell lines were established from adherent cells of the peripheral blood of the bovine which was infected with T.o protozoan parasite. The cell lines were derived from monocytes/macrophages. After detection of T.o in the infected cell lines by the nested-PCR method, the SCID mice were substituted for bovine red blood cells (Bo-RBC-SCID), and transfected with T.o infected cells. As a result, piroplasms were detected in red blood cells in the peripheral blood of Bo-RBC-SCID. These findings suggest that T.o protozoan were subcultured in macrophage cells, differentiated and proliferated into the intraerythrocytic stage after having been infected with the bovine red blood cells in Bo-RBC-SCID.