Genome analysis of Theileria orientalis and its functional genomics

测定了日本牛肠菌病病原菌东方铁杆菌的全基因组序列。基因组总大小为8,983,596 bp，由4条染色体组成。采用寡聚盖法和载体捕集法，结合常规cDNA文库分期，构建了两个全长cDNA文库。共有27478条ESTs被定位到基因组序列上。利用Glimmer 2和Genewise两款基因预测软件，预测了约3900个基因，并对其进行了自动和人工标注。通过与小恙虫和环斑恙虫基因组的比较，发现东方恙虫基因组在结构上存在显著差异。其中，东方滴虫含有单拷贝基因，而在细小滴虫/环孢滴虫中发现的是串联重复的多拷贝基因家族，且每条染色体的亚端粒末端结构不同。通过同源性分析，确定了小孢子虫/环孢虫裂殖体和孢子子蛋白的同源物。此外，还发现了东方蓟带3结合蛋白（TpSCOP）的同源物，并确定了其与肌动蛋白的结合活性及其在宿主细胞耐药凋亡中的可能作用。

The complete genome sequence of Theileria orientalis, the causal agent of bovine theileriosis in Japan, was determined. Total genome size is 8,983,596 bp consisting of four chromosomes. Two full-length cDNA libraries from intraerythrocytic piroplasm were constructed by using oligo-capping and vector-trapper methods and conventional cDNA library stage. A total of 27,478 ESTs were mapped on the genome sequence. By using two gene prediction soft-wares, Glimmer 2 and Genewise, about 3,900 genes were predicted, which were subsequently automatically and manually annotated. By comparison of T. orientalis genome with those of T. parva and T. annulata revealed significant structural differences. Among all, T. orientalis contains single copies of genes which are found as tandem-repeated multiple copy gene families in T. parva/annulata, and structures at subtelomeric ends of each chromosome are different.By synteny analysis, orthologues of T. parva/annulata schizont and spolozoite proteins were identified. Furthermore, orthologue of T. orientalis band 3 binding protein (TpSCOP) was found and its actin-binding activity and possible role in host cell resistance apoptosis were determined.