Genome analysis of Theileria orientalis and its functional genomics

东方泰勒虫基因组分析及其功能基因组学

• 批准号: 17208026
• 负责人: SUGIMOTO Chihiro
• 金额: $ 31.2万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17208026/
• 关键词: Genome; Veterinary Medicine; Microbe; Infectious disease; Protozoa; タイレリア; ショットガン解析; cDNA

The complete genome sequence of Theileria orientalis, the causal agent of bovine theileriosis in Japan, was determined. Total genome size is 8,983,596 bp consisting of four chromosomes. Two full-length cDNA libraries from intraerythrocytic piroplasm were constructed by using oligo-capping and vector-trapper methods and conventional cDNA library stage. A total of 27,478 ESTs were mapped on the genome sequence. By using two gene prediction soft-wares, Glimmer 2 and Genewise, about 3,900 genes were predicted, which were subsequently automatically and manually annotated. By comparison of T. orientalis genome with those of T. parva and T. annulata revealed significant structural differences. Among all, T. orientalis contains single copies of genes which are found as tandem-repeated multiple copy gene families in T. parva/annulata, and structures at subtelomeric ends of each chromosome are different.By synteny analysis, orthologues of T. parva/annulata schizont and spolozoite proteins were identified. Furthermore, orthologue of T. orientalis band 3 binding protein (TpSCOP) was found and its actin-binding activity and possible role in host cell resistance apoptosis were determined.

确定了日本牛泰勒虫病的病原体东方泰勒虫的完整基因组序列。基因组总大小为 8,983,596 bp，由 4 条染色体组成。采用寡加帽和载体捕获方法以及常规cDNA文库阶段构建了两个来自红细胞内梨质的全长cDNA文库。总共 27,478 个 EST 被映射到基因组序列上。通过使用Glimmer 2和Genewise这两款基因预测软件，预测了约3900个基因，并随后进行了自动和手动注释。通过将东方砗磲基因组与T. parva和T. annulata的基因组进行比较，发现了显着的结构差异。其中，东方木蠹含有单拷贝的基因，这些基因在细小蠹/环蠹中被发现为串联重复的多拷贝基因家族，并且每条染色体亚端粒末端的结构都不同。此外，还发现了东方木霉带3结合蛋白（TpSCOP）的直系同源物，并确定了其肌动蛋白结合活性和在宿主细胞抵抗凋亡中的可能作用。

项目成果

PCR-based discrimination of Toxoplasma gondii from pigs at an abattoir Okinawa, Japan.

日本冲绳一家屠宰场基于 PCR 技术对猪中的弓形虫进行鉴别。

• 发表时间: 2006
• 期刊: J. Vet Med Sci. 68(4)
• 作者: Zakimi S;Kyan H;Oshiro M;Sugimoto C;Fujisaki K
• 通讯作者: Fujisaki K

Comparative analysis of heat shock protein 70 genes Babesia parasite isolated from dogs.

犬巴贝斯虫热休克蛋白 70 基因的比较分析

• 发表时间: 2006
• 作者: Yamazaki;M.
• 通讯作者: M.

Development of Highly sensitive and simple diagnostic method for trypanosomiases

开发高灵敏度、简单的锥虫病诊断方法

• 发表时间: 2007
• 作者: Thekisoe;O.
• 通讯作者: O.

Identification of ribosomal phosphoprotein PO of Neospora caninum as a potential vaccine candidate

鉴定犬新孢子虫核糖体磷蛋白 PO 作为潜在候选疫苗

• 发表时间: 2007
• 作者: Thekisoe;O.;工藤京子;Zhang Houshuang
• 通讯作者: Zhang Houshuang

イヌより分離したバベシア原虫由来のheat shock protein 70遺伝子の比較と解析

犬巴贝斯虫原虫70个热休克蛋白基因的比较分析

• 发表时间: 2006
• 作者: 山崎;真大
• 通讯作者: 真大