Studies on the heavy-metal tolerance and interactions between several metal-binding ligands in plants exposed to complex pollution conditions.

研究暴露于复杂污染条件下的植物的重金属耐受性和几种金属结合配体之间的相互作用。

• 批准号: 22580377
• 负责人: INOUHE Masahiro
• 金额: $ 3.08万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580377/
• 关键词: 環境浄化; 植物生理

In the present studies, we examined the possible mechanisms for the heavy-metal tolerance and interactions with metal-binding ligands in plants exposed to complex conditions. We found(1) In tomato cells, phytochelatin (PC) formation and accumulation are involved in the expression of the heavy-metal tolerance characteristics. For the detoxification mechanism, required formation of the PC-complex can be strongly facilitated with Cd but inhibited by other elements such as Cu,As and B, which are not incorporated in the former PC-Cd complex.(2) Transports of Cd and As have been associated with the thiol peptides such as PCs and GSH in literatures. However, their transports can be significantly affected by organic acids and some inorganic such as borate. Under conditions where different metals and anions are mixed in medium, their transport rates from roots to shoots are very different (e.g. Cd and Zn are much faster than Cu or As, but in a manner which is correlated with some organic ligands).Here, borate facilitates the Cd transport but inhibits As transport to shoot.(3) Other major mono- and/or divalent metal ions reveal other unique mechanisms for transport and redistribution with ligands in plants, which taken together present new prospects in futurestudy.

在目前的研究中，我们研究了植物在复杂条件下的重金属耐受性及其与金属结合配体相互作用的可能机制。我们发现(1)在番茄细胞中，植物螯合素(PC)的形成和积累参与了耐重金属特性的表达。在解毒机制上，Cd能强烈促进所需的PC-Cd络合物的形成，而其他元素如铜、As、B等则抑制其形成。(2)Cd和As的转运与文献中发现的PC、GSH等硫醇肽有关。然而，它们的转运会受到有机酸和一些无机物质(如硼酸盐)的显著影响。在不同金属和阴离子混合的条件下，它们从根到地上部的运输速率有很大的不同(例如，Cd和Zn的运输速度比Cd或As快得多，但其运输方式与某些有机配体有关)。在这里，硼酸盐促进Cd的运输，但抑制As向地上部的运输。(3)其他主要的一价和/或二价金属离子揭示了植物体内其他独特的与配体一起运输和再分配的机制，这些都为未来的研究提供了新的前景。

项目成果

Changes in the water status and osmotic solute contents in response to drought and salicylic acid treatments in four different cultivars of wheat (Triticum aestivumL.).

四种不同小麦品种（Triticum aestivumL.）对干旱和水杨酸处理的水分状况和渗透溶质含量的变化。

• 发表时间: 2012
• 期刊: J. Plant Res
• 作者: N. Loutfy;M.A. El-Tayeb;A.M. Hassanen;M.F.M. Moustafa;Y. Sakuma and M. Inouhe.
• 通讯作者: Y. Sakuma and M. Inouhe.

イネの耐塩性と浸透圧調節における適合溶質と無機イオンの相互作用

水稻耐盐性和渗透压调节中相容性溶质与无机离子的相互作用

• 发表时间: 2012
• 作者: 市拓也;佐久間洋;井上雅裕
• 通讯作者: 井上雅裕

Coordination of organic and inorganic solutes in drought and salt tolerance in cereal plants.

谷类植物耐旱和耐盐性中有机和无机溶质的协调。

• 发表时间: 2012
• 作者: Inouhe M.;Ichi T.;Matsumoto A.;Sakuma Y;Naglaa L.
• 通讯作者: Naglaa L.

Roles of phytochelatins in heavy metal stress and detoxification mechanisms in plants

植物螯合素在植物重金属胁迫和解毒机制中的作用

• 发表时间: 2013
• 作者: Tomotaka Tanabe;Tatsuya Funahashi;Katsushiro Miyamoto;Hiroshi Tsujibo;and Shigeo Yamamoto;S. Chatterjee
• 通讯作者: S. Chatterjee

Roles of phytochelatin and other biological ligands in heavy metal detoxifications and transports in tomato plants and suspension cells.

植物螯合素和其他生物配体在番茄植株和悬浮细胞中重金属解毒和运输中的作用。

• 发表时间: 2012
• 期刊: In Abstract of VIPCA II Conference for Plant Growth, Nutrition and Environment Interaction, Vienna, Austria
• 作者: Inouhe M.;Ichi T.;Matsumoto A.;Sakuma Y.;Loutfy N.;Nakano A.;Kanao T;Inouhe M. and Gupta D.K.
• 通讯作者: Inouhe M. and Gupta D.K.