Identification and functional analysis of chondroitin sulfatesinvolved in sizing and development of eye

参与眼睛大小和发育的硫酸软骨素的鉴定和功能分析

• 批准号: 22590174
• 负责人: UEHARA Mosato
• 金额: $ 2.91万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590174/
• 关键词: 網膜; 眼球; 糖鎖; ニワトリ胚; 眼球発生; 眼圧低下; 色素上皮層; 神経網膜; 眼球の拡張; Pax6; Visinin; 眼球の発生、発達; 眼内圧; 網膜の剥離; 細胞増殖; コンドロイチン硫酸鎖; コンドロイン硫酸プロテオグリカン; アグリカン; ビグリカン; 視神経; 部位・時期特異的分布

We tried to identify chondroitin sulfates closely related sizing and morphogenesis of eye and analyze its function. Chondroitin-4-sulfate and chondoritin-6-sulfate and its proteoglycans were distributed in the retina throughout the developmental periods. Especially, decorin, one of the chondroitin sulfate proteoglycans, were present in abundance in the entire eyeball. Injecting anti-decorin antibody in vitreous cavity on embryonic day 5.5 (HH stage 28), the expansion of chick embryo eye was significantly suppressed. However, proliferation and cell proliferation of retical cells were occurred regardless ofthe suppression of eye expansion. The sclera was also thickened and inhibited eye expansion. These results indicated that decorin involved in the determination of retinal formation and eyeball volume and sizing through the regulation of the expansion of the vitreous body.

本研究试图鉴定与眼大小和形态发生密切相关的硫酸软骨素，并对其功能进行分析。软骨素-4-硫酸酯和软骨素-6-硫酸酯及其蛋白多糖在整个发育时期的视网膜中分布。尤其是硫酸软骨素蛋白多糖之一核心蛋白聚糖，在整个眼球中大量存在。在鸡胚5.5天（HH 28期）玻璃体腔内注射抗核心蛋白聚糖抗体，鸡胚眼的扩张受到明显抑制。而视网膜细胞的增殖和细胞增殖与眼球扩张的抑制无关。巩膜也增厚并抑制眼球扩张。这些结果表明核心蛋白聚糖通过调节玻璃体的膨胀参与视网膜的形成和眼球的体积和大小的决定。

项目成果

Expression of Small Leucine-Rich Proteoglycans in the Developing Retina and Kainic Acid-Induced Retinopathy in ICR Mice

• DOI: 10.1292/jvms.10-0464
• 发表时间: 2011-04-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Ali, Safwat Ali Mohamed;Hosaka, Yoshinao Z.;Uehara, Masato
• 通讯作者: Uehara, Masato

発生早期の眼圧低下が眼球拡張と網膜形成に及ぼす影響

早期降低眼压对扩瞳和视网膜形成的影响

• 发表时间: 2013
• 作者: 保坂善真;田中智子;上原正人
• 通讯作者: 上原正人

ニワトリ胚眼球の拡張と形態形成に及ぼす眼内圧の影響

眼压对鸡胚眼球扩张及形态发生的影响

• 发表时间: 2012
• 作者: Tanaka Tomoko;Hosaka Yoshinao Z;Uehara Masato;田中智子
• 通讯作者: 田中智子

Effect of intraocular pressure on expansion and morphogenesis in chick embryo eye

眼压对鸡胚眼扩张和形态发生的影响

• 发表时间: 2012
• 作者: Tanaka Tomoko;Hosaka Yoshinao Z;Uehara Masato
• 通讯作者: Uehara Masato

Spatiotemporal Distribution of Chondroitin Sulfate Proteoglycans in the Developing Mouse Retina and Optic Nerve

• DOI: 10.1292/jvms.10-0201
• 发表时间: 2011-01-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Ali, Safwat Ali Mohamed;Hosaka, Yoshinao Z.;Uehara, Masato
• 通讯作者: Uehara, Masato