Biological analyses of scavenger receptor CL-P1 which is a host defense factor

宿主防御因子清道夫受体 CL-P1 的生物学分析

• 批准号: 22590259
• 负责人: OHTANI Katsuki
• 金额: $ 2.5万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-10-20 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590259/
• 关键词: 発生・分化; コレクチン; スカベンジャー受容体; 血管内皮細胞; ノックアウトマウス; トランスジェニックマウス; 胎生致死; 自然免疫

I have been working for the elucidation of the role in the biological and developmental processes of CL-P1. Since the CL-P1 gene knockout mice are embryonic lethal, I examined the lethal time, it was clarified that it is pre-implantation. I have studied a function of CL-P1 after the fertilization using the transgenic mouse which developed GFP by the promoter of the CL-P1 gene, but I was able to confirm the expression from immediately after fertilization, but it was not possible to clarify whether play what function. And creating conditional knockout mice in order to analyze the role in vivo, it was examined, but did not provide a new finding at the present stage. I continue examining it and would like to try for the elucidation of the biological CL-P1 in future.

我一直在努力阐明Cl-P1的生物学和发育过程中的作用。由于Cl-P1基因敲除小鼠是胚胎致死的，因此我检查了致命的时间，因此可以澄清它是植入前的。我使用Cl-P1基因启动子开发GFP的转基因小鼠在受精后研究了Cl-P1的功能，但我能够立即从受精后立即确认表达，但无法阐明是否发挥作用。并创建有条件的敲除小鼠以分析体内的角色，但在当前阶段没有提供新发现。我继续研究它，并希望将来阐明生物CL-P1。

项目成果

Gene regulation function of the three specificity protein-1 (Sp1) within the human collectin placenta-1 proximal promoter

• DOI: 10.1007/s13258-011-0001-9
• 发表时间: 2011-06-01
• 期刊: GENES & GENOMICS
• 影响因子: 2.1
• 作者: Kim, Youn Uck;Ohtani, Katsuki;Wakamiya, Nobutaka
• 通讯作者: Wakamiya, Nobutaka

Molecular cloning and functional analysis of scavenger receptor zebrafish CL-P1

• DOI: 10.1016/j.bbagen.2011.09.016
• 发表时间: 2011-12-01
• 期刊: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
• 影响因子: 3
• 作者: Fukuda, Mitsuko;Ohtani, Katsuki;Wakamiya, Nobutaka
• 通讯作者: Wakamiya, Nobutaka

Collectin CL-P1 is an endocytosis and phagocytosis receptor using clathrin-dependent endocytosis pathway and clathrin-independent phagocytosis pathway

Collectin CL-P1 是一种利用网格蛋白依赖性内吞途径和网格蛋白非依赖性吞噬途径的内吞和吞噬受体

• 发表时间: 2013
• 作者: Wakamiya N;Jang SJ;Fukuoh A;Mori K;Suzuki Y;Ohtani K
• 通讯作者: Ohtani K

Novel collectins play double roles in embryonic and fetal development as well as innate immunity

新型集合素在胚胎和胎儿发育以及先天免疫中发挥双重作用

• 发表时间: 2013
• 期刊: Seikagaku
• 作者: Ohtani K;Wakamiya N
• 通讯作者: Wakamiya N

Collectin and scavenger receptor CL-P1 is an endocytosis receptor using clathrin-dependent endocytosis pathway and clathrin-independent phagocytosis pathway

集合素和清道夫受体 CL-P1 是一种利用网格蛋白依赖性内吞途径和网格蛋白非依赖性吞噬途径的内吞受体

• 发表时间: 2013
• 作者: Wakamiya N;Jang SJ;Fukuoh A;Mori K;Suzuki Y;Ohtani K
• 通讯作者: Ohtani K