Studies on dynamics of plasmacytoid dendritic cells by gene-targeting technology.

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In order to track plasmacytoid dendritic cells (pDCs) in vivo, we generated SiglecH-fluorescent protein and SiglecH-cre recombinase knock-in mice, in which genes for a fluorescent protein and a cre recombinase were knocked into the gene locus of a SiglecH expressed highly in pDC. In SiglecH-fluorescent protein knock-in mice, fluorescent-positive cells were detected not only in the majority of pDC, but also in a small population of CD8+ dendritic cells in spleen and Peyer's patches. We observed all of the blood cells analyzed were tdRFP-positive in SiglecH-cre: Rosa-loxP-STOP-loxP-tdRFP mice, which carry cre-expressing tdRFP-positive cells. These data suggest that we cannot label and track pDCs in vivo by use of these knock-in mice. I propose that we choose other genes highly and specifically expressed in pDCs, and generate new knock-in mouse line to label pDCs in vivo.