Research on solubilization of citrullinated fibrinogen and fibrin

我推测类风湿关节炎患者血液和关节液中存在的瓜氨酸纤维蛋白原(C-FBG)和纤维蛋白(C-FBN)在抗瓜氨酸化蛋白抗体(ACPA)存在下，可以避免某些酶的降解，发挥长效免疫原性，并提供炎症区域。为了证明我的理论，我制作了C-FBG和C-FBN，并与蛋白酶反应。在ACPA存在下，C-FBG和C-FBN分别与正常FBG和正常FBN相似地被纤溶酶、中性粒细胞弹性蛋白酶、类胰蛋白酶、糜酶或基质金属蛋白酶-3消化。总而言之，C-FBG和C-FBN的蛋白酶消化期并没有延长，这与我的预期相反。

I speculated that citrullinated fibrinogen (C-Fbg) and fibrin (C-Fbn) existed in blood and joint fluid in patients with Rheumatoid Arthritis were avoided the degradation by some of proteases in the presence of anti-citrullinated protein antibody (ACPA), exerted long acting immunogenic property, and provided inflammatory region. To demonstrate my theory, I made a C-Fbg and C-Fbn, and reacted with proteases. In the presence of ACPA, C-Fbg and C-Fbn were digested similar to normal Fbg and normal Fbn, respectively, with plasmin, neutrophil elastase, tryptase, chymase, or MMP-3. In conclusion, elongation of protease digestion period for C-Fbg and C-Fbn were not observed in contrary to my expectation.