Research on solubilization of citrullinated fibrinogen and fibrin

我推测类风湿关节炎患者血液和关节液中存在瓜氨酸化纤维蛋白原（C-Fbg）和纤维蛋白（C-Fbn），在抗瓜氨酸化蛋白抗体（ACPA）存在的情况下，避免了部分蛋白酶的降解，发挥了长期免疫原性，提供了炎症区。为了证明我的理论，我制作了C-Fbg和C-Fbn，并与蛋白酶反应。在ACPA存在的情况下，C-Fbg和C-Fbn分别被纤溶酶、中性粒细胞弹性酶、胰蛋白酶、切酶或MMP-3消化，类似于正常Fbg和正常Fbn。综上所述，没有观察到C-Fbg和C-Fbn的蛋白酶消化期延长，这与我的预期相反。

I speculated that citrullinated fibrinogen (C-Fbg) and fibrin (C-Fbn) existed in blood and joint fluid in patients with Rheumatoid Arthritis were avoided the degradation by some of proteases in the presence of anti-citrullinated protein antibody (ACPA), exerted long acting immunogenic property, and provided inflammatory region. To demonstrate my theory, I made a C-Fbg and C-Fbn, and reacted with proteases. In the presence of ACPA, C-Fbg and C-Fbn were digested similar to normal Fbg and normal Fbn, respectively, with plasmin, neutrophil elastase, tryptase, chymase, or MMP-3. In conclusion, elongation of protease digestion period for C-Fbg and C-Fbn were not observed in contrary to my expectation.