Characterization of bacterial biota in the distal esophagus of Japanese patients with reflux esophagitis and Barrett's esophagus

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We investigated bacterial composition in the distal esophagus in subjects with a normal esophagus, reflux esophagitis, and Barrett’s esophagus. Biopsy specimens were obtained from the distal esophagus and used for histological examination and DNA extraction. Fragments of 16S rDNA genes were amplified by PCR using general bacterial primers, and bacterial populations were examined. Microbial communities differed among patients with a normal esophagus, reflux esophagitis and Barrett’s esophagus. From this study, we conclude that esophageal bacterial composition differs under conditions of normal esophagus, reflux esophagitis, and Barrett’s esophagus and Diverse bacterial communities may be associated with esophageal disease.