Analysis of in vivo effects of possible immune regulatory moleculesfor artificial management of immune systems

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We analyzed in vivo effects of signal-transducing adaptor protein-2 (STAP-2) as well as proliferin, pleiotrophin, and osteoblast stimulating factor (OSF)-5. We established knock-in chimera mice to produce proliferin, pleiotrophin, or OSF-5, respectively. Only OSF-5-KI chimera mice showed lymphocytopenia in peripheral blood, spleen, and bone marrow. The mice had few B lymphocytes after pre-B cell differentiation. In addition, OSF-5 was produced by stromal cells. We also evaluated DSS-induced colitis in signal-transducing adaptor protein-2 (STAP-2)-knockout mice. STAP-2 deficiency resulted in less body weight loss and colon shortening. Colon cells had responsibility to less phenotypes of colitis, and production of chemokines was impaired in STAP-2-knockout mice.