Development of Early Detection kit for diagnosis mucormycosis

毛霉菌病的诊断依赖于组织病理学鉴定和培养确认。然而，培养通常没有生长，具有典型毛霉菌结构的生物体的组织病理学鉴定可能提供感染的唯一证据。临床医生必须在适当的临床环境中考虑这种实体，并进行侵入性检查，以便尽早确定诊断。因此，需要新的早期检测方法。采用一种新的信号序列诱捕方法对米根霉蛋白抗原进行筛选。两个未知的具有信号序列的蛋白，抗原A （26kDa，假设蛋白）和B （46kDa，假设蛋白）被选为候选蛋白。抗原A的检测采用ELISA试剂盒进行。抗原A在R. oryzae、R . micro孢子、R . micro孢子var. rhizopodiformis的培养上清中检测。在根霉感染小鼠血清中也检测到该抗原。

The diagnosis of mucormycosis relies upon the identification of organisms in tissue by histopathology with culture confirmation. However, culture often yields no growth, and histopathologic identification of an organism with a structure typical of Mucorales may provide the only evidence of infection. A clinician must think of this entity in the appropriate clinical setting and pursue invasive testing in order to establish a diagnosis as early as possible. Therefore, new early detection methods are required. We used a novel signal sequence trap methods to select protein antigen from Rhizopus oryzae. Two unknown proteins with signal sequences, antigen A (26kDa, hypothetical protein) and B (46kDa, hypothetical protein) were selected as candidates. We made ELISA kit to detect antigen A. The antigen A was detected in the culture supernatant of R. oryzae、R microspores、R microspores var. rhizopodiformis. The antigen was also detected in the serum form Rhizopus infected mice.