Neural tube defect manifestation due to the interaction of genetic mutation and Ochratoxin A in the genetic polydactyly/ arhinencephaly mouse embryo, Pdn/Pdn

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Ochratoxin A (OTA) is a teratogen causing neural tube defects (NTDs) in mice. We analyzed the gene expression by using the genetic polydactyly/arhinencephaly mouse (Pdn/Pdn) to examine the pathogenesis of NTDs. After treatment with 2 mg/kg of OTA to Pdn/+ female mice, mated with Pdn/+ male, on day 7.5 of gestation, gene expressions in the embryo were analyzed by DNA microarray, real-time PCR and whole mount in situ hybridiyzation (WISH) on day 9 of gestation. From these investigations, it was suggested that NTDs induced by OTA may be the result of altered multiple expressions in Pdn/Pdn embryos.