Creation of atopic dermatitis model mouse using double knock out mouse

利用双基因敲除小鼠建立特应性皮炎模型小鼠

• 批准号: 22591240
• 负责人: YONEDA Kozo
• 金额: $ 3.33万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591240/
• 关键词: ロリクリン; ノックアウトマウス; 魚鱗癬; アトピー性皮膚炎; バリア; フレーキーテールマウス; フィラグリン

Atopic dermatitis is one of intractable skin diseases. The clinical feature of this disease is itchy eczema. This disease is caused by mutations in filaggrin gene now. Loricrin is major constituent of marginal band of epidermal corneocyte. The clinical aspects of the variant form of Vohwinkel syndrome are mutilating keratoderma with ichthyosis. This phenotype is similar to ichthyosis vulgaris. Ichthyosis vulgaris is caused by homozygous or compound heterozygous mutations in the filaggrin gene. The aim of this project is creation of atopic dermatitis mouse model using loricrin knock out mouse.

特应性皮炎是难治性皮肤病之一。这种疾病的临床特征是瘙痒性湿疹。目前认为该病是由聚丝蛋白基因突变引起的。兜甲蛋白是表皮角质细胞边缘带的主要成分。Vohwinkel综合征变异型的临床表现是毁损性角化病伴鱼鳞病。这种表型与寻常型鱼鳞病相似。寻常性鱼鳞病是由聚丝蛋白基因的纯合或复合杂合突变引起的。本课题的目的是利用兜甲蛋白基因敲除小鼠建立特应性皮炎小鼠模型。

项目成果

Detection of apoptotic keratinocytes in a case of bullous pemphigoid developed after graft-versus-host disease.

• DOI: 10.2340/00015555-1676
• 发表时间: 2014-03
• 期刊: Acta dermato-venereologica
• 影响因子: 3.6
• 作者: K. Yoneda;T. Demitsu;M. Kakurai;T. Narita;K. Nakai;Y. Kubota;N. Ishii;T. Hashimoto

Two cases of nevus sebaceous accompanying secondary tumors with Klothov expression

皮脂腺痣伴发Klothov表达继发性肿瘤2例

• DOI: 10.1111/1346-8138.12371
• 发表时间: 2014
• 期刊: J Dermatol
• 影响因子: 3.1
• 作者: Nakai K;Yoneda K;Haba R;Kushida Y;Katsuki N;Moriue J;Moriue T;Ishikawa E;Inoue S and Kubota Y
• 通讯作者: Inoue S and Kubota Y

Abnormally aggregated caveolin-1 in the cytoplasm of cellular model of classic Vohwinkel syndrome

经典 Vohwinkel 综合征细胞模型细胞质中异常聚集的 Caveolin-1

• 发表时间: 2010
• 作者: Yoneda K;Nakai K;Moriue T;Demitsu T and Kubota Y
• 通讯作者: Demitsu T and Kubota Y

Sphingosine 1‐phosphate attenuates peroxide‐induced apoptosis in HaCaT cells cultured in vitro

1-磷酸鞘氨醇减弱过氧化物诱导的体外培养 HaCaT 细胞凋亡

• 发表时间: 2013
• 期刊: Clincal and Experimental Dermatology
• 作者: T. Moriue;J. Igarashi;K. Yoneda;T. Hashimoto;K. Nakai;H. Kosaka;Y. Kubota
• 通讯作者: Y. Kubota

Consequences of two different amino-acid substitutions at the same codon in KRT14 indicate definitive roles of structural distortion in epidermolysis bullosa simplex pathogenesis

KRT14 中同一密码子的两个不同氨基酸取代的结果表明结构扭曲在单纯性大疱性表皮松解症发病机制中的明确作用

• 发表时间: 2011
• 期刊: J Invest Dermatol
• 影响因子: 6.5
• 作者: K.Natsuga;W.Nishie;et al.
• 通讯作者: et al.