Measuring human complement activation for antibody mediated rejection

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In transplantation, various antibodies cause antibody mediated rejection. The clinical significance of HLA antibodies depends on their ability to activate complement (C). The requirements of HLA antibodies to activate human C are not known. Direct C3b measurement on target cells may by a better indication of the ability of HLA antibodies to activate human C than standard cytotoxicity. Human C3b deposition was influenced more by the particular serum/cell combination under study than by the amount of IgG, with some combinations showing high IgG and low C3b and others showing low IgG and high C3b. IVIG produced significant dose-dependent complement inhibition. Measuring human C component may improve the assessment of donor-recipient compatibility. IVIG is useful for reducing antibody mediated rejection.