Proteomic analysis of cyst fluid associated with renal cell carcinoma

肾细胞癌相关囊液的蛋白质组学分析

• 批准号: 22591776
• 负责人: IWAMURA Masatsugu
• 金额: $ 2.83万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591776/
• 关键词: 腎細胞癌; 腫瘍マーカー; ペプチド解析; プロテオーム解析; 嚢胞液; アガロース2次元電気泳動; HPLC; 14-3-3 protein; ペプチド; プロテオーム

Proteins in the cyst fluid were separated by reverse-phase high- performance liquid chromatography and agarose two- dimensional gel electrophoresis and were identified by tandem mass spectrometry. Our search resulted in the identification and selection of 14-3-3 protein beta/alpha, which was overexpressed in cyst fluid from cyst-associated RCC but has not been previously associated with RCC. The expression levels of 14 -3-3 protein beta/ alpha were higher in urine samples from patients with RCC than in samples from healthy volunteers, suggesting that 14-3-3 Protein beta/ alpha may be a diagnostically useful biomarker for early diagnosis of RCC.

囊液中的蛋白质通过反相高效液相色谱和琼脂糖双向凝胶电泳进行分离，并通过串联质谱进行鉴定。我们的搜索导致了14-3-3蛋白β/α的鉴定和选择，该蛋白在囊肿相关RCC的囊液中过表达，但以前与RCC无关。14 - 3 - 3蛋白β/α在肾细胞癌患者尿液中的表达水平高于健康志愿者，提示14-3-3蛋白β/α可能是肾细胞癌早期诊断的有用生物标志物。

项目成果

Laparoscopic radical nephrectomy:points of technique

腹腔镜肾癌根治术：技术要点

• 作者: Matsushima M;Kikuchi E;Maeda T;Nakashima J;Sugawara A;Ando T;Mizuno R;Nagata H;Miyajima A;Shigematsu N;Oya M;M.Iwamura
• 通讯作者: M.Iwamura

Cytocidal effects of acridine orange evoked by blue light on human bladder cancer cells

蓝光激发吖啶橙对人膀胱癌细胞的杀伤作用

• 发表时间: 2012
• 期刊: Kitasato Med J
• 作者: Morihiro Nishi;HIromi Hiruma;Haruko Sasamoto;Masatsugu Iwamura;Risa Isonaka;Shiro Baba
• 通讯作者: Shiro Baba

小径腎腫瘍に対する腹腔鏡下腎部分切除術後腎機能に関する臨床的検討―無阻血・阻血手術の比較―

腹腔镜小直径肾肿瘤肾部分切除术后肾功能的临床研究-非缺血性手术与缺血性手术的比较-

• 发表时间: 2012
• 期刊: Japanese Journal of Endourology and ESWL
• 作者: 西盛宏;岩村正嗣;藤田哲夫;笹本治子;山田泰史;馬場志郎
• 通讯作者: 馬場志郎

Approach of the lower urinary system

下泌尿系统的入路

• 发表时间: 2012
• 作者: T.Piechaud;CC.Abbou;R.Van Velthoven;S.Egawa;M.Iwamura,SH.Lu;YC.Tsai;SM.Wang
• 通讯作者: SM.Wang

Cystotomy

膀胱切开术

• 发表时间: 2011
• 作者: Ide H;Tokiwa S;Sakamaki K;Nishio K;Isotani S;Muto S;Hama T;Masuda H.;Horie S;上村博司;Masatsugu Iwamura
• 通讯作者: Masatsugu Iwamura