Proteomic analysis of cyst fluid associated with renal cell carcinoma

囊液中的蛋白质通过反相高效液相色谱和琼脂糖双向凝胶电泳进行分离，并通过串联质谱进行鉴定。我们的搜索导致了14-3-3蛋白β/α的鉴定和选择，该蛋白在囊肿相关RCC的囊液中过表达，但以前与RCC无关。RCC患者尿液样本中14 - 3 - 3蛋白β/α的表达水平高于健康志愿者样本，这表明14-3-3蛋白β/α可能是早期诊断RCC的诊断有用的生物标志物。

Proteins in the cyst fluid were separated by reverse-phase high- performance liquid chromatography and agarose two- dimensional gel electrophoresis and were identified by tandem mass spectrometry. Our search resulted in the identification and selection of 14-3-3 protein beta/alpha, which was overexpressed in cyst fluid from cyst-associated RCC but has not been previously associated with RCC. The expression levels of 14 -3-3 protein beta/ alpha were higher in urine samples from patients with RCC than in samples from healthy volunteers, suggesting that 14-3-3 Protein beta/ alpha may be a diagnostically useful biomarker for early diagnosis of RCC.