New Biomarkers for Lymph Node Metastasis in Patients with EndometrialCancer using Exon-expression Microarray

点击翻译按钮获取中文摘要

To predict lymph node metastasis using primary cancer tissue preoperatively, we explored putative biomarker genes of node metastasis in patients with endometrial cancer. Three up-regulated transcripts in node-positive group were picked up; ANKRD36 (ankyrin repeat domain containing 36), CROP and MALAT-1. mmunostaining analyses elucidated that ANKRD36 and CROP are localized in cytoplasm and nuclei of cancer cells, respectively. Furthermore, in node-positive group, ANKRD36 protein expression levels weresignificantly high compared with one in node-negative group. In situ hybridization showedMALAT-1 was localized in nucleus of carcinoma cells. Further analyses will be performed to clarify biological function of these 3 molecules which might be the putative node-positive biomarkers in endometrial cancer.