The analysis of novel molecular targeted therapy inhibiting S100A4 for ovarian cancer

我们发现S100A4基因启动子到内含子之间-4~248位区域的低氧反应元件(HRE)是其在卵巢癌细胞中过表达的重要原因。因此，认为低氧环境可以上调S100A4的表达。接下来，我们利用包含10,000种化合物的化合物文库进行了S100A4基因转录抑制化合物的筛选。我们发现有6种化合物对其基因转录有明显抑制作用。这些化合物被认为是新型分子靶向治疗的候选化合物。

We revealed that the Hypoxia Response Element(HRE) existing in -4 to +248 on the region from promoter to intron in S100A4 gene was important for its over-expression in ovarian carcinoma cells. Thus, low oxygen environment was considered to up-regulate the expression of S100A4. Next, we performed the screening of compounds suppressing the transcription of S100A4 gene using compounds library containing 10,000 kinds of compound. We found 6 kinds of compound significantly suppressing the transcription of its gene. These were regarded as a candidate compound for novel molecular targeted therapy.