Analysis for hematopoiesis and hematologic diseases by L3MBTL1 conditional knockout mice.

L3MBTL1条件敲除小鼠的造血功能和血液疾病分析。

• 批准号: 22790903
• 负责人: ARAI Satoko
• 金额: $ 2.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790903/
• 关键词: 造血; クロマチン制御; 血液疾患

It has been supposed that dysfunction of L3MBTL1 may cause hematological diseases. In this study, we propose the construction of L3MBTL1-conditional knock out mouse in which L3MBTL1 can be deleted specifically in the hematopoietic cells to know the function of L3MBTL1 in hematopoiesis. Unfortunately, we could not accomplish generation of the mutant mice. In addition, I performed the pull-down assay by using Halotag-fusion L3MBTL1 protein expressed in human leukemic cell lines to clarify the L3MBTL1-conmplex in hematopoietic cells, and the candidates for the associated proteins were obtained.

有研究认为，L3MBTL1功能障碍可能导致血液病的发生。本研究建议构建L3MBTL1条件性基因敲除小鼠，其中L3MBTL1可以在造血细胞中特异性缺失，以了解L3MBTL1在造血中的作用。不幸的是，我们未能完成突变小鼠的世代。此外，我还利用在人白血病细胞系中表达的Halotag-Fusion L3MBTL1蛋白进行了下拉实验，以明确L3MBTL1-在造血细胞中的复合体，并获得了相关蛋白的候选者。