Roles of XB130, a novel adaptor protein, in cell cycle progression of gastric cancer cells via regulation of c-Myc

XB130（一种新型接头蛋白）通过调节 c-Myc 在胃癌细胞周期进程中的作用

• 批准号: 22791295
• 负责人: SHIOZAKI Atsushi
• 金额: $ 2.58万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791295/
• 关键词: アダプター蛋白; 胃癌; 食道癌; 細胞周期; 胃十二指腸外科学

Adaptor proteins can participate in the regulation of various cellular functions, depending on the functional role of their interacting partners. We have cloned a novel adaptor protein, XB130, which has been implicated as a substrate and regulator of tyrosine kinase-mediated signaling and in controlling cell proliferation and apoptosis in thyroid and lung cancer cells. However, its expression and role in gastrointestinal cancer have not been investigated. In the present study, we sought to determine the role of XB130 in gastric and esophageal cancer cells and examine its expression and effects on the prognosis of patients with upper gastrointestinal cancer. Using knockdown experiments with XB130 siRNA, we found that XB130 regulate cell cycle progression via c-Myc and p21 in gastric and esophageal cancer cells. Immunohistochemical analysis revealed that expression of XB130 in cancer tissue was observed in 71.2% of patients with esophageal cancer, and that XB130 expression in the nucleus was an independent prognostic factor. The present study leads to the discovery of XB130 as an important prognostic factor in upper gastrointestinal cancer, and has the potential to present as a novel mediator and/or biomarker.

衔接蛋白可以根据其相互作用伙伴的功能作用来参与各种细胞功能的调节。我们克隆了一种新型的衔接蛋白XB130，该蛋白被认为是酪氨酸激酶介导的信号传导以及控制甲状腺和肺癌细胞中细胞增殖和凋亡的底物和调节剂。但是，尚未研究其在胃肠道癌中的表达和作用。在本研究中，我们试图确定XB130在胃癌和食道癌细胞中的作用，并检查其对上胃肠道癌患者预后的表达和影响。使用XB130 siRNA的敲低实验，我们发现XB130在胃癌和食管癌细胞中通过C-Myc和P21调节细胞周期进程。免疫组织化学分析表明，在71.2％的食管癌患者中观察到XB130在癌症组织中的表达，而核中​​XB130的表达是独立的预后因素。本研究导致发现XB130是上胃肠道癌的重要预后因素，并且有可能作为新型介体和/或生物标志物出现。

项目成果

Prediction of CCND1 amplification using plasma DNA as a prognostic marker in oesophageal squamous cell carcinoma.

• DOI: 10.1038/sj.bjc.6605657
• 发表时间: 2010-04-27
• 期刊: British journal of cancer
• 影响因子: 8.8

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纵隔气气法食管切除术病例研究

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気縦隔法による食道切除術,50例の治療経験と有効性の検討

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気縦隔法による食道切除術54例の治療経験とその有効性の検討

纵隔气气法食管切除术54例治疗体会及疗效检验

• 发表时间: 2011
• 作者: 塩崎敦、藤原斉、大辻英吾;ら
• 通讯作者: ら

HALS+気縦隔法を用いた食道切除術～30例の治療経験～

HALS+纵隔气气法食管切除术～30例治疗经验～

• 发表时间: 2011
• 作者: 塩崎敦、藤原斉、大辻英吾;ら
• 通讯作者: ら