Molecular roles of xCT and XB130 in esophageal squamous cell carcinoma

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The aims of the present study were to determine the roles of xCT, cysteine/glutamate transporter in esophageal squamous cell carcinoma (ESCC) cells, its clinical significance and interaction with XB130, adaptor protein. Knocking-down (KD) of xCT decreased XB130 expression, and KD of XB130 increased xCT expression in ESCC cells. Immunoprecipitation revealed no interaction between xCT and XB130. In TE13 and KYSE170 cells, KD of xCT inhibited G1-S phase progression. Microarray analysis identified 1652 genes whose expression levels in TE13 cells were altered by the KD of xCT. Pathway analysis showed that the top-ranked pathway was the G1/S checkpoint regulation. Immunohistochemical staining showed that the xCT positivity rate was positively correlated with the Ki-67 labeling index. Further, its expression was an independent prognostic factor. These observations suggest that the expression of xCT in ESCC cells might affect the cell cycle and impact on the prognosis of ESCC patients.