Analysis of anti-nociceptive mechanism induced by gamma knife irradiation for intractable pain

伽玛刀照射治疗顽固性疼痛的抗伤害机制分析

• 批准号: 22791358
• 负责人: YAGASAKI Yuki
• 金额: $ 3.16万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791358/
• 关键词: 機能脳神経外科学; ガンマナイフ; 難治性疼痛; 下垂体; マクロファージ; 慢性炎症性疼痛; 慢性神経因性疼痛; Folliculostellate cell; 神経科学; 鎮痛

Gamma knife pituitary radiosurgery (GKPR) is used to manage cancer pain and has provided marked effects. However, the analgesic mechanism of GKPR remains unclear. We established a GKPR method for rats. Since pituitary atrophy was observed after high-dose GKPR, we confirmed that the GK irradiation had been correctly performed on the rat pituitary. Next, we determined the effects of 120Gy GK irradiation, a dose similar in energy to actual treatment, on the pituitary. However, no behavioral changes related to nociceptive responses were observed after GK irradiation of pituitary in the normal rat. Immunoblotting and histochemical analysis showed that Iba-1 positive cells (macrophage/microglia) were significantly increased at the GK treated pituitary. Further studies, such as GK irradiation of the pituitary in a chronic inflammatory pain model, may clarify the mechanisms underlying the anti-nociceptive effects induced by GK.

伽玛刀垂体放射外科（GKPR）用于治疗癌症疼痛，并提供了显着的效果。然而，GKPR的镇痛机制仍不清楚。我们建立了大鼠GKPR方法。由于大剂量GKPR后观察到垂体萎缩，我们证实GK照射已正确地对大鼠垂体进行。接下来，我们确定了120 Gy GK照射（能量与实际治疗相似的剂量）对垂体的影响。而正常大鼠垂体经GK照射后，未见与伤害性反应有关的行为学改变。免疫印迹和组织化学分析表明，Iba-1阳性细胞（巨噬细胞/小胶质细胞）在GK处理的垂体显着增加。进一步的研究，如在慢性炎性疼痛模型中对垂体的GK照射，可能阐明GK诱导的抗伤害性作用的机制。

项目成果

Iba-1 positive macrophage is activated in the anterior pituitary in the chronic inflammatory pain model rat.

在慢性炎性疼痛模型大鼠的垂体前叶中，Iba-1 阳性巨噬细胞被激活。

• 发表时间: 2014
• 作者: 矢ヶ崎有希;片山洋子;長田知也;木下陽子;川上順子
• 通讯作者: 川上順子

Establishment of the gamma knife pituitary radiosurgery for a rat : Study for management of intractable pain with gamma knife

大鼠伽玛刀垂体放射外科手术的建立：伽玛刀治疗顽固性疼痛的研究

• 作者: 矢ヶ崎有希;片山洋子;木下陽子;林基弘;田村徳子;長田知也;川上順子
• 通讯作者: 川上順子

Histological analysis of pituitary gland in acute and chronic pain rat models

急慢性疼痛大鼠模型垂体的组织学分析

• 发表时间: 2011
• 作者: 片山洋子;木下洋子;矢ヶ崎有希;川上順子
• 通讯作者: 川上順子

Amygdala-prefrontal pathways and the dopamine system affect nociceptive responses in the prefrontal cortex.

• DOI: 10.1186/1471-2202-12-115
• 发表时间: 2011-11-15
• 期刊: BMC neuroscience
• 影响因子: 2.4
• 作者: Onozawa K;Yagasaki Y;Izawa Y;Abe H;Kawakami Y
• 通讯作者: Kawakami Y

Gamma knife irradiation of injured sciatic nerve induces histological and behavioral improvement in the rat neuropathic pain model.

• DOI: 10.1371/journal.pone.0061010
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Yagasaki Y;Hayashi M;Tamura N;Kawakami Y
• 通讯作者: Kawakami Y