Identifying the origin and niche environment of nucleus pulposus progenitor cells of the intervertebral disc and its therapeutic applications

椎间盘髓核祖细胞的起源和生态位环境的识别及其治疗应用

• 批准号: 22791406
• 负责人: SAKAI Daisuke
• 金额: $ 2.5万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791406/
• 关键词: 整形外科学; 脊椎脊髄病学; 椎間板; 再生医療; 幹細胞; 再生医学; 髄核細胞; 脊椎; ニッチ; 椎間板変性

Despite many reports on the identification of endogenous stem/progenitor cell populations in various musculoskeletal tissues, no definitive cell population has been reported in NP tissue. Furthermore, the origin or niche that regulates the self-renewal and maintenance of NP stem/progenitor cells is also unknown. The primary goal of the current study was to identify a set of specific markers to isolate the cell population sharing universal stem cell characteristics from the NP. Then we sought to determine the presence of a stem-cell niche in NP tissue and the role of NP stem cells in ageing and degeneration of the IVD in humans. Results demonstrated that in mouse and human nucleus pulposus cell populations, isolated by defined surface markers possess stem cell characteristics, such as clonogenicity, self-renewal and multi-potent differentiation ability in vitro, and tissue maintenance ability in vivo.

尽管有许多关于在各种肌肉骨骼组织中鉴定内源性干/祖细胞群的报道，但在NP组织中没有明确的细胞群报道。此外，调节NP干/祖细胞自我更新和维持的起源或小生境也是未知的。本研究的主要目的是鉴定一组特异性标记物，以从NP中分离具有通用干细胞特征的细胞群。然后，我们试图确定NP组织中干细胞龛的存在以及NP干细胞在人类IVD老化和退化中的作用。结果表明，在小鼠和人髓核细胞群中，通过确定的表面标记分离的细胞具有干细胞特性，如体外克隆形成、自我更新和多潜能分化能力，以及体内组织维持能力。

项目成果

Effects of a Glycogen Synthase Kinase-3β Inhibitor (LiCl) on c-myc Protein in Intervertebral Disc Cells

• DOI: 10.1002/jcb.23217
• 发表时间: 2011-10-01
• 期刊: JOURNAL OF CELLULAR BIOCHEMISTRY
• 影响因子: 4
• 作者: Hiyama, Akihiko;Sakai, Daisuke;Mochida, Joji
• 通讯作者: Mochida, Joji

Biological treatments : cells. how do they work

生物治疗：细胞。

• 发表时间: 2009
• 作者: Daisuke Kubota;Yoshiyuki Suehara;Akira Kawai and Tadashi Kondo;et al;澤地恭昇;Sakai D
• 通讯作者: Sakai D

椎間板髄核幹/前駆細胞、その培養方法および用途

椎间盘髓核干/祖细胞、其培养方法和用途

• 发表时间: 2010

Enhancement of intervertebral disc cell senescence by WNT/β-catenin signaling-induced matrix metalloproteinase expression.

• DOI: 10.1002/art.27599
• 发表时间: 2010-10
• 期刊: ARTHRITIS AND RHEUMATISM
• 作者: Hiyama, Akihiko;Sakai, Daisuke;Risbud, Makarand V.;Tanaka, Masahiro;Arai, Fumiyuki;Abe, Koichiro;Mochida, Joji
• 通讯作者: Mochida, Joji

軟骨再生の基礎と臨床の進歩(椎間板を含む)椎間板再生細胞移植療法のその先に

软骨再生（包括椎间盘）的基础知识和临床进展超越椎间盘再生细胞移植疗法

• 发表时间: 2010
• 作者: 酒井大輔;持田讓治
• 通讯作者: 持田讓治